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Simple Method for Simultaneous Removal of Multiple Impurities from Culture Supernatants to Ultralow Levels

a technology of culture supernatants and impurities, applied in the direction of separation processes, peptides/protein ingredients, peptides, etc., can solve the problems of media nutrients and components contributing contaminants, media nutrients contributing contaminants, and affecting so as to reduce the level of contaminants or undesired impurities to ultralow levels, enhance the purity of the target, and reduce the level of contaminants

Active Publication Date: 2011-10-27
SERUM INST OF INDIA PTE LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0009]The present invention overcomes the problems and disadvantages associated with current strategies and designs, and provides new tools and methods for simultaneous removal of multiple contaminants from culture supernatants, cell extracts, plant extracts or crude lysate. The method may also be applied to partially purified products to reduce the contaminants or undesired impurities to ultralow levels. Ultralow levels of impurities are levels that are preferably from 91% to 99.9% reduced as compared to unpurified substances. The invention may also be used in conjunction with additional purification steps to further enhance the purity of the target and reduce the level of contaminants.

Problems solved by technology

In addition to biosynthetic products produced during fermentation, media nutrients and components also contribute contaminants.
In addition to biosynthetic products produced during fermentation, media nutrients also contribute contaminants.
Product purification typically involves multiple and complex steps to reduce impurity levels to acceptable levels.
Multiple and complex steps adversely affect yield, quality, stability, processing time and process operations.
Further, these processes are expensive to run, require a high degree of skill to perform, and a significant amount of time to reduce impurity levels.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Meningococcal C Polysaccharide

[0037]Capsular polysaccharide was derived from bacterial fermentation. The culture supernatant containing products was obtained by centrifugation or cross flow filtration of fermentation broth.

Anion Exchange Chromatography Sorbent: Q-Sepharose

Buffers:

[0038]Equilibration Buffer (20 mM Phosphate buffer pH 7.5)[0039]Wash Buffer-I (15% IPA+0.25% Triton X-100+0.3 M NaCl, pH 7.5)[0040]Wash buffer-II (6 M Urea+0.25% Triton X-100+0.3 M NaCl, pH 7.5)[0041]Wash Buffer III (20 mM phosphate buffer+0.3 M NaCl, pH 7.5)[0042]Elution buffer (20 mM Phosphate buffer, 0.5 M NaCl, pH 7.5)[0043]Regeneration Buffer (1 M NaCl and 0.5 M NaOH)

[0044]A column with 20 cm inner diameter (Pharmacia, BPG 200 / 500) was packed according to the manufacturer instructions using 6 L Pharmacia Q Sepharose Fast flow sorbent with a fixed bed height of ˜200 mm. The column was washed with 10 column volumes (CV) of water for Injection (WI) and then charged and sanitized using 5 CV of 1 M sodium c...

example 2

Haemophilus influenzae Type b (Nib) Polysaccharide

[0052]The culture supernatant containing product was obtained by centrifugation or cross flow filtration of fermentation broth to separate the bacterial cells. Supernatant obtained containing polysaccharide with impurities was processed through one step method of purification.

Anion exchange chromatography: Sorbent: Q-Sepharose

Buffers:

[0053]Equilibration Buffer (20 mM Phosphate buffer pH 7.5)[0054]Wash Buffer-I (15% IPA+0.25% Triton X-100+0.3 M NaCl, pH 7.5)[0055]Wash buffer-II (6M Urea+0.25% Triton X-100+0.3 M NaCl, pH 7.5)[0056]Wash Buffer III (20 mM phosphate buffer+0.3 M NaCl, pH 7.5)[0057]Elution buffer (20 mM Phosphate buffer, 0.5 M NaCl, pH 7.5)[0058]Regeneration Buffer (1 M NaCl and 0.5 M NaOH)

[0059]A column with 20 cm inner diameter (Pharmacia, BPG 200 / 500) was packed according to the manufacturer instructions using 6 L Pharmacia Q Sepharose Fast flow sorbent with a fixed bed height of ˜200 mm. The column was washed with 10 c...

example 3

Recombinant Erythropoietin Protein

[0068]The culture supernatant containing product was obtained by cross flow filtration of fermentation broth to separate the cells. Supernatant obtained containing protein with media components and impurities was processed through one step method of purification. The culture used was recombinant mammalian cell CHO cell line for erythropoietin production.

Hydrophobic interaction chromatography: Sorbent: TOYOPEARL® (Toyopearl-butyl; Tosohas)

Buffers:

[0069]Equilibration Buffer (20 mM Phosphate buffer pH 7.2, 0.75 M NaCl)[0070]Wash buffer (19% IPA+0.75 M NaCl, pH 7.2)[0071]Elution buffer (27% IPA+0.75 M NaCl, pH 7.2)[0072]Regeneration Buffer (1 M NaOH)

[0073]A column with 20 cm inner diameter (GE, BPG 200 / 500) was packed according to the manufacturer instructions using 6 L GE Butyl Sepharose sorbent with a fixed bed height of ˜200 mm. The column was washed with 10 column volumes (CV) of water for Injection (WEI) and then sanitized using 5 CV of 0.5 M NaOH ...

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PUM

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Abstract

This invention is directed to methods for removing, preferably simultaneously and in one step, multiple impurities form crude culture samples, and, in particular, the removal of media components, protein, nucleic acids, lipids, and lipopolysaccharides to ultralow levels. Preferably the purification process comprises: (1) binding of the target substance containing one or more contaminants to a chromatography matrix; (2) washing the bound target substance with one or more buffers containing a synergistic combination of a lyotropic agent or organic solvent, a detergent, and a salt component; and (3) desorbing the target substance from the chromatography matrix, so that the eluate contains ultra low levels of contaminants. The reduction of impurities that can be achieved is preferably 91-99.9% as compared to the amount of impurities in the target substance before purification. The invention is also directed to the targets products that have been so purified.

Description

REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority to U.S. Provisional Application No. 61 / 327,238 of the same title filed Apr. 23, 2010, the entirety of which is hereby incorporated by reference.BACKGROUND[0002]1. Field of the Invention[0003]This invention is directed to a method for simultaneously removing, preferably in one step, multiple impurities from crude sample containing products produced by cell culture or fermentation and, in particular, the removal of contaminants such as media components, proteins, nucleic acids, lipids, and lipopolysaccharides to ultralow levels. The product may be produced by yeast, bacterial or mammalian cells with impurities reduced to ultralow level of 91% to 99.9% as compared to the purified target substance. The invention is also directed to the products that have been purified according to this method.[0004]2. Description of the Background[0005]Polysaccharides, proteins and nucleic acids are synthesized by various organisms ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07K1/22C07K14/34C07K14/505C08B37/00C07K1/18
CPCC07K1/16C07K1/18C07K1/20B01D15/426C07K14/34C07K14/505B01D15/00C07K1/22
Inventor LEES, ANDREWJOSHI, JAYANT SAKARAM
Owner SERUM INST OF INDIA PTE LTD
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