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Lateral flow assays

a technology of lateral flow and assays, applied in the field of lateral flow assays, can solve the problems of complex not being labeled and nothing being detectable at the test lin

Inactive Publication Date: 2011-04-14
RAPID PATHOGEN SCREENING INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0008]Embodiments of the present invention include assays where the analyte (target) to be detected does not bind directly to an immobilized binding partner in the test zone of a test strip. Instead, the analyte preferably interacts with one

Problems solved by technology

However, the resulting complex is not labeled and nothing is detectable at the test line.

Method used

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Embodiment Construction

[0033]Rapid point-of-care analysis is becoming increasingly important in the screening and / or diagnosis and treatment of various viral and other pathogenic microbiological agents. Prior art point-of-care tests, such as lateral flow immunochromatography tests, are immunoassays involving an antibody and its antigen. Prior art sandwich immunoassays included at least one binding partner for the analyte immobilized at the test line in the detection zone of the test strip. Binding assays in some formats operate on the basis of ligands and receptors and their associated binding constants.

[0034]The inherent deficiencies in the associations between antibodies and their antigens are well known in the art in that ligand-receptor binding assays are prone to degrade with temperature cycling and heat stress while in storage, and interferences often occur from components in the sample matrix, causing non-specific binding during the assay and leading to false results. These inherent limitations do ...

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Abstract

Assays and methods including mobile tagged single stranded nucleic acid reagents pre-loaded on an analysis device, which are preferably tagged, but not labeled and are complementary to a strand (preferably the anti-sense strand in double stranded DNA targets) of the target nucleic acid. The assay also includes a running buffer that includes a dye or other detectable label that nonspecifically binds only to double stranded nucleic acids. In addition, the analysis device includes a detection zone including one or more test zones that have an immobilized tag that binds to the tag on the mobile nucleic acid reagent.

Description

REFERENCE TO RELATED APPLICATIONS[0001]This application claims one or more inventions which were disclosed in Provisional Application No. 61 / 266,641, filed Dec. 4, 2009, entitled “LATERAL FLOW NUCLEIC ACID DETECTOR”, Provisional Application No. 61 / 331,966, filed May 6, 2010, entitled “MULTIPLANAR LATERAL FLOW ASSAY WITH SAMPLE COMPRESSOR”, Provisional Application No. 61 / 352,093, filed Jun. 7, 2010, entitled “LATERAL FLOW ASSAYS”, and Provisional Application No. 61 / 392,981, filed Oct. 14, 2010, entitled “MULTIPLANAR LATERAL FLOW ASSAY WITH SAMPLE COMPRESSOR”.[0002]This application is also a continuation-in-part application of application Ser. No. 12 / 502,626, filed Jul. 14, 2009, entitled “LATERAL FLOW NUCLEIC ACID DETECTOR”, which claims one or more inventions which were disclosed in Provisional Application No. 61 / 080,879, filed Jul. 15, 2008, entitled “LATERAL FLOW NUCLEIC ACID DETECTOR”, Provisional Application No. 61 / 098,935, filed Sep. 22, 2008, entitled “1N SITU LYSIS OF CELLS I...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/50
CPCC12Q1/6834G01N33/5308Y10T436/143333C12Q2565/625C12Q2565/519C12Q2525/161
Inventor BABU, UMA MAHESHSAMBURSKY, ROBERT P.VANDINE, ROBERT W.
Owner RAPID PATHOGEN SCREENING INC
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