Multipotent Adult Stem Cells And Uses of Multipotent Adult Stem Cells To Treat Inflammation

Abstract

Disclosed are cell preparations comprising multipotent adult stem cells and methods for using multipotent adult stem cells to treat autoimmune diseases, treat allergic responses, treat cancer, treat inflammatory diseases, treat fibrotic disorders, reduce inflammation and / or fibrosis, promote would healing, repair epithelial damage, and / or promote angiogenesis.

Description

RELATED APPLICATIONS[0001]This application is a continuation of application Ser. No. 11 / 541,853, filed Oct. 2, 2006, which is a continuation-in-part of application Ser. No. 11 / 080,298, filed Mar. 15, 2005, which claims priority based on provisional application Ser. No. 60 / 555,118, filed Mar. 22, 2004; the contents of each application are hereby incorporated by reference in their entireties.FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT[0002]The present technology was made with Government support under Contract No. N66001-02-C-8068 awarded by the Department of the Navy. The Government has certain rights in this technologyBACKGROUND OF THE INVENTION[0003]Mesenchymal stem cells (MSCs), which are present in adult bone marrow, are a kind of multipotent stem cell that can differentiate readily into lineages including osteoblasts, myocytes, chondrocytes, and adipocytes. (Pittenger, et al., Science, Vol. 284, pg. 143 (1999); Haynesworth, et al., Bone, Vol. 13, pg. 69 (1992); Prockop, Science, ...

AI Technical Summary

Benefits of technology

[0006]In accordance with the present technology, multipotent adult stem cells can be used, for example, to treat an autoimmune disease, treat an inflammatory response, treat an allergic disease, treat a pulmonary disease having fibrotic and / or inflammatory components, repair epithelial damage, and promote wound healing in subjects, including a human subject. Autoimmune diseases that can be treated with multipotent adult stem cells include, for example, Type 1 Diabetes, inflammatory bowel disease, Crohn's disease, and uveitis. Pulmonary diseases that can be treated with multipotent adult stem cells include, but are not limited to, Acute Respiratory Distress Syndrome (ARDS), Chronic Obstructive Pulmonary Disease (COPD), and asthma. Inflammatory responses, including those associated with autoimmune diseases or pulmonary diseases, can be reduced with multipotent adult stem cells. An inflammatory response can be reduced by, for example, reducing the production or expression of pro-inflammatory mediators, increasing the production or expression of anti-inflammatory mediators, or a combination thereof.

[0009]The present technology provides one or more cell preparations comprising adult bone marrow-derived stem cells in one or more doses effective to treat an inflammatory response in a subject. The subject can be a human having, for example, inflammatory bowel disease. The stem cells can be capable of differentiating into at least one cell type of each of the endodermal, ectodermal, and mesodermal embryonic lineages. The effective dose of the cell preparation can contain a sufficient number of stem cells to provide about 1×105 to about 1×107 cells per kilogram of the subject. In certain embodiments, the subject can be a mammal. The mammal can be, for example, a primate, including a human and a non-human primate. Administration of the cell preparation can, for example, elevate interferon-beta levels in the subject.

[0016]Furthermore, it is believed that multipotent adult stem cells stimulate dendritic cells (DCs) to produce Interferon-Beta (IFN-β), which promotes tumor suppression and immunity against viral infection.

Problems solved by technology

These immunological properties of MSCs can enhance their transplant engraftment and limit the ability of the recipient's immune system to recognize and reject allogeneic cells following transplantation.

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