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Scavenger of in vivo harmful reactive oxygen species and/or free radicals

a technology of reactive oxygen species and free radicals, which is applied in the direction of anti-noxious agents, packaged goods types, metabolic disorders, etc., can solve the problems that both of these vitamins cannot free radicals are harmful, and agents cannot always easily reach the internal regions of cells, so as to reduce oxidative stress, suppress aging process, and reduce damage

Inactive Publication Date: 2009-02-05
OHTA SHIGEO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0019]The present inventors have considered that hydrogen molecules in a gas state are taken up into living bodies and rapidly distributed into cells, and such hydrogen molecules can be expected to eliminate free radicals at a point in time when the free radicals are generated. Thus, the present inventors have conducted various studies and have consequently completed the present invention by confirming that hydrogen molecules can alleviate damage caused by free radicals in cells; hydrogen molecules are actually taken up into the bodies of animals including humans; and hydrogen actually alleviates oxidative stress in the bodies of animals.
[0020]Specifically, an object of the present invention is to provide a scavenger of free radicals, which is capable of effectively reducing the concentrations of in vivo free radicals and exhibiting given effects such as the suppression of aging process, the prevention of geriatric or lifestyle-related diseases, health promotion, and the inhibition of oxidative stress by virtue of this reduction in the concentrations of free radicals, and to provide an apparatus for the inhalation of this scavenger of free radicals.
[0021]O2−. or hydrogen peroxide with a low concentration functions as a signal molecule for controlling a large number of signal transduction cascades, in spite of its cytotoxicity, and has physiologically important functions such as the control of physiological processes, for example, apoptosis, cell growth, and cell differentiation. Hydrogen peroxide with a high concentration is converted to hypochlorous acid by myeloperoxidase, and this hypochlorous acid exhibits antimicrobial effects. Furthermore, nitrogen monoxide radicals, which are neurotransmitters, play an important role in vasodilation. By contrast, radicals having unilateral cytotoxicity, such as .OH, can be neutralized by hydrogen molecules without inhibiting basic physiological activities possessed by reactive oxygen species as described above. The present inventors have completed the present invention by finding that hydrogen molecules can alleviate .OH-induced cytotoxicity without influencing other reactive oxygen species, and hydrogen molecules have the potential of being applicable as an antioxidant to medical care.

Problems solved by technology

Therefore, both of these vitamins cannot easily reach the internal regions of cells.
Meanwhile, not only are free radicals harmful, but also they have been demonstrated to play roles indispensable to living bodies.
However, these agents cannot always easily reach the internal regions of cells.
Alternatively, even if the reductive water as disclosed in Patent Documents 1 to 4 above is drank, it is obvious that this reductive water is not capable of widely penetrating into the internal and external regions of cells, with its reducing properties maintained.
In addition, the structures and components of living bodies are complicated and are not homogeneous systems.
Therefore, the effects of the reductive water cannot easily be predicted.

Method used

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  • Scavenger of in vivo harmful reactive oxygen species and/or free radicals
  • Scavenger of in vivo harmful reactive oxygen species and/or free radicals
  • Scavenger of in vivo harmful reactive oxygen species and/or free radicals

Examples

Experimental program
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Effect test

example 1

[0121]Hydrogen molecules have been known to have reducing power. However, it is impossible to predict, as described above, whether hydrogen molecules can reduce, in a short time, molecular species participating in biologically important oxidation-reduction, and free radical molecules. Thus, in Example 1, hydrogen water was used to measure effects on each molecular species.

Preparation of Hydrogen Water

[0122]First, 1 l of water was injected into a 5-l pressure-resistant bottle (manufactured by UNICONTROLS. CO., LTD.). Then, hydrogen gas was charged thereinto at a pressure of 0.4 MPa. After 2 hours, water supplemented with hydrogen was collected from the bottle under reduced pressure. A hydrogen content was analyzed using a dissolved hydrogen measuring apparatus (manufactured by ABLE & Biott Co., Ltd.). Water containing approximately 0.8 mM saturated hydrogen (hydrogen water) was obtained by this method.

Measurement of Reactive Oxygen Species Eliminating Effects of Hydrogen Water

[0123]S...

example 2

[0131]Antimycin A, which is an inhibitor of mitochondrial respiratory chain complex III, promotes reactive oxygen species production in cells and induces oxidative stress-induced cell death as a result. Thus, in Example 2, the defensive effects of hydrogen against oxidative stress in the presence of antimycin A was measured.

Preparation of Hydrogen-Containing Medium

[0132]A cell culture medium requires containing oxygen, being almost neutral, and containing no metal ions with a high concentration. For preparing a culture medium containing oxygen and hydrogen molecules coexisting with each other, it can be predicted to separately dissolve these molecules under pressure according to the Henry's law. The space in the hydrogen pressure device is filled with oxygen. Then, hydrogen gas is compressed to 5 atm. The partial pressure of oxygen gas can be kept at 1 atm. Therefore, an oxygen concentration necessary for culture can be secured. Penicillin G (manufactured by Invitrogen) at a final c...

example 3

Examination of Inhibitory Effects of Hydrogen on Cell Death Induced by Menadione

[0138]Menadione, which is an inhibitor of mitochondrial respiratory chain complex I, promotes reactive oxygen species production in cells and induces oxidative stress-induced cell death as a result. Thus, to measure the defensive effects of hydrogen against oxidative stress as in the experiment on antimycin A-induced cell death shown in Example 2, PC12 cells were cultured in a collagen-coated 24-well cell culture dish. Then, 2-ml of the hydrogen-containing medium supplemented or unsupplemented with menadione (manufactured by Sigma) at varying concentrations was added to each well. After 24 hours, the number of viable cells having a pyramid-shaped cell form was counted under a phase contrast microscope. In this experiment, a hydrogen-free medium was used as a comparative control. The results are shown in FIG. 3. The bar graph shows an average of at least 4 wells, and the error bars denote standard deviati...

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Abstract

An object of the present invention is to provide a scavenger of in vivo harmful reactive oxygen species and / or free radicals, which is capable of effectively reducing the concentrations of in vivo reactive oxygen species and / or free radicals and exhibiting given effects such as the suppression of aging process, the prevention of geriatric or lifestyle-related disease, health promotion, and the inhibition of oxidative stress by virtue of this reduction in the concentrations of reactive oxygen species and / or free radicals. The scavenger of in vivo harmful reactive oxygen species and / or free radicals of the present invention comprises a liquid or gas comprising at least a hydrogen molecule. This medium may further comprise an oxygen molecule. Furthermore, this medium may comprise water or an aqueous solution or may be a gas. The scavenger of reactive oxygen species and / or free radicals can be used in the treatment or prevention of a disorder attributed to reactive oxygen species and / or free radicals.

Description

TECHNICAL FIELD[0001]The present invention relates to a scavenger of reactive oxygen species and / or free radicals, which reduces the concentrations of in vivo harmful reactive oxygen species and / or free radicals. Particularly, the present invention relates to a scavenger of in vivo harmful reactive oxygen species and / or free radicals, which is capable of effectively reducing the concentrations of in vivo reactive oxygen species and / or free radicals and exhibiting effects such as the suppression of aging process, the prevention of geriatric or lifestyle-related diseases, health promotion, and the protection against oxidative stress.BACKGROUND ART[0002]It is now accepted widely that the presence of reactive molecules called reactive oxygen species or free radicals is generally at least one of causes of many human health abnormalities including aging, cancers, atherosclerosis, myocardial infarction, attacks, viral infections, lung abnormalities, bowel diseases, and neurodegenerative di...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61P39/06A61K33/00B65D85/00A61M16/10
CPCA23L1/30A23L2/52A23L3/3427A61K33/00A61K2300/00A23L33/10A61P3/00A61P3/06A61P9/00A61P9/10A61P21/00A61P25/00A61P25/16A61P25/28A61P27/02A61P29/00A61P35/00A61P39/06
Inventor OHTA, SHIGEOMUROTA, WATARUOHSAWA, IKUROH
Owner OHTA SHIGEO
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