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Yeast cells expressing modified g proteins and methods of use therefor

a technology of g protein and g protein, which is applied in the field of g protein expressing yeast cells and methods of use therefor, can solve the problems of particular challenges for modulators of these receptors, coupling and functional integration, and achieve enhanced heterologous expression of receptors and enhanced coupling of heterologous expressed gpcrs to g protein subunits.

Inactive Publication Date: 2008-10-30
CADUS TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0010]The present invention provides an important advance in drug screening methodologies previously known in the art by providing, inter alia, a means by which expression of heterologously expressed receptors is enhanced and a means by which coupling of heterologously expressed GPCRs to G protein subunits is enhanced.
[0020]In preferred embodiments, a yeast cell of the present invention comprises a heterologous G protein coupled receptor which is functionally integrated into the yeast cell. In particularly preferred embodiments, a modified or chimeric G protein subunit of the present invention demonstrates enhanced coupling to a heterologous G protein coupled receptor expressed by a yeast cell when compared to that demonstrated by an endogenous yeast G protein subunit.

Problems solved by technology

However, due to the complexity of GPCRs the search for modulators of these receptors have presented particular challenges.
The use of yeast cells for such expression provides advantages in terms of the ease of manipulating the cells, but also presents particular challenges in achieving efficient coupling and functional integration.

Method used

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  • Yeast cells expressing modified g proteins and methods of use therefor
  • Yeast cells expressing modified g proteins and methods of use therefor
  • Yeast cells expressing modified g proteins and methods of use therefor

Examples

Experimental program
Comparison scheme
Effect test

example 1

Construction of Chimeric Yeast / Mammalian Gα Subunits

[0262]This Example pertains to the construction of chimeric G protein subunits which are made using a first polypeptide derived from a yeast G protein subunit and a second polypeptide derived from a mammalian G protein subunit. The Gα subunit of heterotrimeric G proteins interacts with both the βγ complex and the receptor. The construction of chimeric α subunits can, therefore, enhance receptor coupling. In this example, various G protein subunit chimeras were constructed using a polypeptide derived from yeast GPA1 and several different mammalian Gα subunits. The nucleotide sequence of GPA1 is known in the art. For example, the gene encoding a G protein homolog of S. cerevisiae was cloned independently by Dietzel and Kurjan (supra) (who referred to the gene as SCG1) and by Nakafuku, et al. (1987 Proc Natl Acad Sci 84:2140-2144) (who called the gene GPA1). The nucleotide sequence of the human G alpha subunits Gαs, Gαi2, Gαi3 also ar...

example 2

Identification of Critical Regions of Gα and Improvement of the Interaction Of Mammalian GαS with Yeast βγ by Mutation

[0272]In this Example, critical regions of various mammalian Gα subunits were identified by making the following series of mutations:

D229S in rat Gαs, S270P in human Gα16, S280P in Gpa1-Gαi2-GαoB and S288P in GPA1-Gα12

[0273]The specificity of coupling of a receptor to a heterotrimeric G-protein is largely determined by the α subunit of the G-protein. This fact has been exploited by expressing human 7 transmembrane receptors in yeast and functionally coupling them into the yeast pheromone response pathway, as a path to drug discovery. In order to accomplish this goal mammalian Gα subunits were expressed in yeast cells whose own pheromone-responsive Gα subunit (GPA1), has been disrupted. As the predominant role of GPA1 is to bind to and sequester the effector-signaling βγ component of the heterotrimer, the effector activity of the mammalian Gα is irrelevant; the activi...

example 3

Construction of STE18 / Mammalian Gγ Chimeric Proteins

[0300]Chimeric proteins comprising STE18, the wild type Gγ subunit of yeast, were also made. The wild type STE18 nucleotide sequence is available in the art. The carobxy terminal 34 amino acids of STE18 are as follows:

GYPVAGSNHFIEGLKNAQKNSQMSNSNSVCCTLM(SEQ ID NO: 29)

The wild type human Gγ nucleotide sequences are available in the art. The carboxy terminal 24 amino acids of human Gγ are as follows:

DPLLTPVPASENPFREKKFFCAIL(SEQ ID NO: 30)

The underlined residues shown are conserved among all of the mammalian Gγ subunits

[0301]The STE18-mammalian Gγ chimeras were constructed using standard molecular biology techniques. The following is a list of the Gγ chimeras which were generated, all are modified forms of yeast STE 18 which comprise a portion of a Gγ subunit. The sequences shown below represent a carboxy terminal alignment of the sequences. The two dots before the first amino acid shown in each of the sequences in the following list i...

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Abstract

The present invention pertains to novel yeast cells which are useful for the expression of heterologous G protein coupled receptors. The yeast cells of the present invention can be used in screening assays which can be used to screen for modulators of G protein coupled receptors. Specifically, the invention provides novel yeast cells which express a heterologous G protein coupled receptor and mutant and / or chimeric G protein subunit molecules which serve to functionally integrate the heterologous into the pheromone signaling pathway of the yeast cell. The invention also provides for the expression of heterologous G protein coupled receptors which are functionally integrated into the yeast cell membrane using a yeast α factor leader sequence. Drug discovery assays using the subject yeast cells are also provided.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation-in-part of U.S. Ser. No. 08 / 689,172, filed on Aug. 6, 1996 now pending, which is a continuation-in-part of U.S. Ser. No. 08 / 582,333, filed Jan. 17, 1996, now pending, which is a continuation-in-part of U.S. Ser. No. 08 / 463,181, filed Jun. 5, 1995, now pending, which is a continuation-in-part of U.S. Ser. No. 08 / 322,137, filed Oct. 13, 1994, now pending, which is a continuation-in-part of U.S. Ser. No. 08 / 309,313, filed Sep. 20, 1994, now abandoned, which is a continuation-in-part of U.S. Ser. No. 08 / 190,328, filed Jan. 31, 1994, now abandoned, which is a continuation-in-part of U.S. Ser. No. 08 / 041,431, filed Mar. 31, 1993, now abandoned, the specifications of which are hereby incorporated by reference.BACKGROUND OF THE INVENTION[0002]Cell surface receptors are an important class of proteins involved in cellular functioning because they are the primary mediators of cell to cell communication. G protein c...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/569C12N1/16C40B30/04C07K14/395C07K14/47C12N15/81G01N33/50
CPCC07K14/395C07K14/4722C07K14/705C07K14/723C07K2319/00C07K2319/02C12N1/16C12N15/81C40B30/06G01N33/50G01N33/566G01N2333/726G01N2500/02
Inventor FOWLKES, DANA M.BROACH, JAMES R.MANFREDI, JOHN P.PAUL, JEREMY I.TRUEHEART, JOSHUAKLEIN, CHRISTINE A.MURPHY, ANDREW J.M.
Owner CADUS TECH
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