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Method and system for molecular array scanner calibration

a technology of molecular array and scanner, applied in the field of molecular array scanner, can solve the problem of elusive methods for precisely calibrating molecular arrays

Inactive Publication Date: 2006-01-26
CORSON JOHN F +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

Ensures accurate and consistent calibration across molecular array scanners, maintaining precise signal intensity measurements by compensating for dye instability and spectral variations, thereby ensuring reliable data collection.

Problems solved by technology

Unfortunately, methods for precisely calibrating molecular arrays have been elusive.

Method used

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  • Method and system for molecular array scanner calibration
  • Method and system for molecular array scanner calibration
  • Method and system for molecular array scanner calibration

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Embodiment Construction

[0038] One embodiment of the present invention provides a means for calibrating molecular array scanners to a reference molecular array scanner and subsequently calibrating the molecular array scanners to maintain the initial calibration. This two-step calibration method uses a first reference array coated with the same dye that is subsequently used in probe molecules and scanned by the molecular arrays during data collection and uses as a second reference array coated with a more stable dye that does not degrade significantly over repeated scans.

[0039]FIG. 9 shows an abstract representation of the excitation and emission-detection components of a molecular array scanner. In FIG. 9, a laser source 902 generates a coherent light beam of a particular wavelength 904 that may be optically filtered and focused by filtering and focusing components 906 to impinge on a small region 908 of the surface of a molecular array 910. Excited target molecules hybridized to the surface of the molecu...

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Abstract

A method and system for calibrating molecular arrays to a reference molecular array, and for subsequently calibrating the molecular arrays to maintain a constant signal-intensity-to-label-concentration ratio. In the first step of the two-step calibration method, a reference array coated with the fluorophore or chromophore used to label probe molecules is employed, while in the second step of the two-step method, a reference array coated with a stable dye is employed.

Description

[0001] This application claims priority to copending application Ser. No. 10 / 086,932, filed Feb. 28, 2002, under 35 U.S.C. 120, the entirety of which is incorporated herein by reference.TECHNICAL FIELD [0002] The present invention is related to molecular array scanners and, in particular, to a method and system for calibrating molecular array scanners. BACKGROUND OF THE INVENTION [0003] The present invention is related to acquisition of molecular-array data and other types of genetic, biochemical, and chemical data from molecular arrays by molecular array scanners. A general background of molecular-array technology is first provided, in this section, to facilitate discussion of the scanning techniques described in following sections. [0004] Array technologies have gained prominence in biological research and are likely to become important and widely used diagnostic tools in the healthcare industry. Currently, molecular-array techniques are most often used to determine the concentrat...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G06F19/00G01N33/20G01N21/27G01N21/64
CPCG01N21/278G01N21/6428Y10T436/10G01N21/6456G01N21/274G01N21/6452
Inventor CORSON, JOHN F.DORSEL, ANDREAS N.PARKER, RUSSELL A.CHOW, ANDRE B.
Owner CORSON JOHN F
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