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One-step enzyme transformation method for producing L-malic acid

An enzyme conversion method and malic acid technology, applied in the field of enzyme engineering, can solve the problems of low conversion rate of production process, low product content of conversion liquid, long separation and coupling time, etc., to improve reaction efficiency, enhance market competitiveness, and improve product quality. high quality effect

Inactive Publication Date: 2007-07-11
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the conversion rate of L-malate pro-immobilized enzyme production process is low, the production process is long, the product content in the conversion solution is low, the consumption of water, electricity, and labor is large, and the post-extraction cost is high, causing the price to be too high, making L- Sales of malic acid are difficult to compete with DL-malic acid and citric acid
Invention patent "new method for producing L-malic acid by enzymatic method" patent number 95111164.7, the patented method takes a long time for enzymatic reaction, separation and coupling, which increases the production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Embodiment 1, in 30L biological reaction crystallizer, load 1kg fumaric acid enzyme liquid, add calcium fumarate 25kg, potassium dihydrogen phosphate 0.0001kg, reaction temperature is 30 ℃, carry out enzyme reaction and separation coupling, after 26 hours The average conversion rate of the substrate fumarate is 99.1%, and then it is separated and purified to prepare L-malic acid. The prepared L-malic acid is tested to meet the standards of the United States Pharmacopoeia.

Embodiment 2

[0010] Embodiment 2, in 100L biological reaction crystallizer, load 1kg fumaric acid enzyme liquid, add calcium fumarate 50kg, magnesium sulfate 0.0005kg, reaction temperature is 45 ℃, carry out enzymatic reaction and separation coupling, after 18 hours, bottom The average conversion rate of fumarate was 99.5%, and then separated and purified to produce 49.75kg of L-malic acid. The prepared L-malic acid is tested to meet the standards of the United States Pharmacopoeia.

Embodiment 3

[0011] Embodiment 3, 1kg fumaric acid enzyme solution is loaded into the biological reaction crystallizer of 50L, add calcium fumarate 38kg, magnesium sulfate 0.0003kg, reaction temperature is 38 ℃, carry out enzymatic reaction and separation coupling, after 23 hours, bottom The average conversion rate of fumarate is 99.4%, and then it is separated and purified to prepare L-malic acid. The prepared L-malic acid is tested to meet the standards of the United States Pharmacopoeia.

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PUM

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Abstract

The invention discloses a manufacturing method of L-malic acid in the enzyme engineering domain, which comprises the following steps: loading fumarase liquid in the biological reacting crystallizer according to weight; adding calcium fumarate and enzyme activity adjuster in the crystallizer with fumarase liquid under normal pressure at 30-45 deg. c; setting the weight rate of fumarase liquid, fumarate and enzyme activity adjuster at 1 25-50 0.0001-0.0005; separating; selecting the enzyme activity adjuster from one or composition of magnesium sulfate, potassium dihydrogen phosphate and zinc sulfate; coupling; reacting for 18-26h; obtaining the crystal of L-malic acid; separating; purifying; obtaining the product.

Description

technical field [0001] The invention relates to a method for producing L-malic acid by a one-step enzyme conversion method, which relates to a process for producing L-malic acid by enzymatic method, and belongs to the field of enzyme engineering. Background technique [0002] L-malic acid is a product with a broad market in industry, food and other fields. As an important chemical raw material, it is superior to citric acid in food as a sour agent. In other industrial fields, L-malic acid can be used as a pollution-free standard Organic acids are used instead of commonly used sodium tripolyphosphate and sodium aminotriacetate to eliminate the disadvantages of algae caused by washing waste liquid being discharged into rivers. At present, the conversion rate of L-malate pro-immobilized enzyme production process is low, the production process is long, the product content in the conversion solution is low, the consumption of water, electricity, and labor is large, and the post-e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46
Inventor 胡永红欧阳平凯
Owner NANJING TECH UNIV
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