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Biodegradable magnetic nanoparticle, preparation method and application thereof

A technology of magnetic nanoparticles and biodegradation, which is applied in the field of nanomaterials of biotechnology, can solve the problems that magnetic nanoparticles cannot be biodegraded, and the adverse effects of cells, etc. It is easy to achieve trace detection and trace reaction, and the process is simple and speeds up. The effect of reaction time

Inactive Publication Date: 2007-07-11
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] The technical problem to be solved in the present invention is to provide a biodegradable magnetic nanoparticle and its preparation method and application, so as to overcome the defects in the prior art that the magnetic nanoparticle cannot be biodegraded and has adverse effects on the cells it contacts

Method used

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  • Biodegradable magnetic nanoparticle, preparation method and application thereof
  • Biodegradable magnetic nanoparticle, preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0052] The present invention adopts the method for inverse microemulsion, prepares magnetic chitosan nano-composite material, obtains the spherical nano-particle that particle size is about 40nm, concrete implementation method is as follows:

[0053] 1. Weigh 1-100mg of chitosan powder with a deacetylation degree of 80-95% and add it to 1-10mL 1% acetic acid solution, ultrasonically dissolve, add 5-25mg γ-Fe 2 o 3 Particles, uniformly dispersed, as the water phase;

[0054] 2. Take 5-50g of paraffin, add 5-10g of Span, 5-10g of Tween, and stir to obtain a uniform system as the oil phase;

[0055] 3. Add the water phase to the oil phase and stir evenly to obtain a transparent brown water-in-oil type (W / O type) inverse microemulsion;

[0056] 4. Add 1-3mL glutaraldehyde to the system as a cross-linking agent, keep the constant temperature water bath at 25-40°C, and mechanically stir. After the reaction, wash the particles with isopropanol to remove the particle surfactant and ...

Embodiment 2

[0060] Magnetic liposomes were prepared by the classic reverse-phase evaporation method. Dissolve an appropriate amount of soybean lecithin and cholesterol (molar ratio 2:1) in a mixed solvent of methanol-chloroform (1:1), and rotate to evaporate to form a uniform transparent lipid film. After dissolving the lipid film with an appropriate amount of ether, add an appropriate amount of γ-Fe 2 o 3 Particle suspension, after ultrasonic emulsification, carry out the second rotary evaporation, and then carry out ultrasonic homogenization, and finally squeeze the liposome suspension through a 450nm microporous filter membrane to obtain magnetic liposomes, and pass nitrogen protection during the operation .

[0061] Liposomes have a cell-like structure, and when they enter the body, they are mainly swallowed by the reticuloendothelial system to activate the body's autoimmune function and change the distribution of the encapsulated drug in the body. Liposomes are stable in lymph, ent...

Embodiment 3

[0063] Using the same inverse microemulsion method as in Example 1, a magnetic blood albumin nanocomposite material was prepared, and spherical nanoparticles with a particle size of about 50 nm were obtained. When albumin is negatively charged, it can be absorbed by lymph under the mediation of receptors, and the absorption rate is related to the negative charge. Negatively charged albumin can reach the embryonic center of the lymph node and around the follicle, not only has the inhibitory effect on HIV replication itself, but also can be used as a carrier of anti-AIDS drugs.

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Abstract

The invention relates to a method for producing biological degradable magnetic nanometer particles, wherein said particle has corn and frame; the corn layer is magnetic ferric oxide; the frame is natural macromolecule materials as liposome, blood albumin, dope, starch, or chitose; its diameter is 1-100nm; the nanometer particles is prepared by reverse-phase micro emulsion method. The inventive particle has wide application.

Description

technical field [0001] The invention belongs to the field of nanometer materials, in particular to nanometer materials used in biotechnology. Background technique [0002] The basic principle of cell separation technology is to select one or several methods to separate specific target cell populations from mixed cell populations or tissues according to the different physical, chemical, biological and immunophenotypic characteristics of different cell populations. seperate. The main method of initially separating and enriching cells is based on the different specific gravity of the cells, using techniques including speed centrifugation, density gradient centrifugation, etc., but the time is long and the effect is poor. The emergence and development of flow cytometric analysis and sorting technology and monoclonal antibody technology in the middle and late 1970s brought a qualitative leap in cell separation technology. Fluorescence-activated cell sorting (FACS) technology co...

Claims

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Application Information

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IPC IPC(8): A61K47/36A61K47/02
Inventor 沈鹤柏朱晨华
Owner SHANGHAI NORMAL UNIVERSITY
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