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Radix actinidiae argutae extract and its anticanceruse

A technology of root extract and vine pear, applied in the field of vine pear root extract, can solve the problems of a large number of organic solvents, unclear active ingredients, complicated extraction process, etc., and achieve good separation effect and easy operation

Inactive Publication Date: 2007-06-13
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of the existing complicated extraction process, the need to use a large amount of organic solvents, and unclear active ingredients, thereby providing a wisteria root extract with a simple process, suitable for industrial production, and a relatively single active ingredient

Method used

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  • Radix actinidiae argutae extract and its anticanceruse
  • Radix actinidiae argutae extract and its anticanceruse

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Weigh 120g of vine pear root, dissolve it in 480ml of industrial alcohol, reflux for 5 hours, extract once, rotate the extract, and dry to obtain 4.86g of brown-red paste. Weigh 0.1g periana alcohol extract, 30g silica gel powder, and carry out silica gel column chromatography separation. Use dichloromethane, methanol and acetic acid solution for gradient elution, the sequence is: the volume ratio of dichloromethane and methanol is 1:3, 1:5, 1:10, 1:30, pure methanol, methanol and acetic acid Mixed solutions with a volume ratio of 1:2, 1:3, and 1:5. The eluent is continuously eluted, and the eluate of the yellow and light yellow color bands are collected respectively. The solvent was removed by rotary evaporation at 30° C. to obtain 0.045 g and 0.010 g of yellow powders respectively, which were vacuum-dried for 24 hours to obtain medium-polarity and high-polarity wisteria root extracts of the present invention, respectively.

Embodiment 2

[0021] Weigh 200g of vine pear root, dissolve in 1200ml of industrial alcohol, reflux for 10 hours, extract 3 times, combine the extracts, evaporate them by rotary evaporation, and obtain 10.02g of brown-red paste after drying. Weigh 1.05g periana alcohol extract, 420g silica gel powder, carry out silica gel column chromatography separation. Use dichloromethane, methanol and acetic acid solution for gradient elution, the sequence is: the volume ratio of dichloromethane and methanol is 1:3, 1:5, 1:10, 1:30, pure methanol, methanol and acetic acid Mixed solutions with a volume ratio of 1:2, 1:3, and 1:5. The eluent is continuously eluted, and the eluate of the yellow and light yellow color bands are collected respectively. The solvent was removed by rotary evaporation at 40° C. to obtain 0.54 g and 0.15 g of yellow powders respectively, which were vacuum-dried for 24 hours to obtain medium-polarity and high-polarity wisteria root extracts of the present invention, respectively....

Embodiment 3

[0023] Weigh 100 g of vine pear root, dissolve in 500 ml of industrial alcohol, reflux for 8 hours, extract twice, combine the extracts and then rotary evaporate, and dry to obtain 4.68 g of brown-red paste. Weigh 2.00g periana alcohol extract, 500g silica gel powder, and carry out silica gel column chromatography separation. Use dichloromethane, methanol and acetic acid solution for gradient elution, the sequence is: the volume ratio of dichloromethane and methanol is 1:3, 1:5, 1:10, 1:30, pure methanol, methanol and acetic acid Mixed solutions with a volume ratio of 1:2, 1:3, and 1:5. The eluent is continuously eluted, and the eluate of the yellow and light yellow color bands are collected respectively. The solvent was removed by rotary evaporation at 40° C. to obtain 0.98 g and 0.29 g of yellow powders respectively, which were vacuum-dried for 24 hours to obtain medium-polarity and high-polarity wisteria root extracts of the present invention, respectively.

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Abstract

The present invention relates to a Chinese gooseberry root extract. Its preparation method includes the following steps: (1), extraction: adding ethyl alcohol in the gooseberry root, making reflux extraction, combining extracts, making rotary evaporation, drying to obtain brown paste material; (2), separation and collection: using mixture of dichloromethane, ethyl alcohol and acetic acid as eluant; making the above-mentioned brown paste material be passed through silica-gel chromatographic column to make elution and separation; respectively collecting eluents of yellow color band and pale yellow color band; and (3), removing solvent: respectively removing solvent from every eluent, vacuum drying, respectively obtaining the invented semi-polar gooseberry root extract and strong-polar gooseberry root extract. Said gooseberry root extracts can be used for preparing medicine for curing carcinoma.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a wisteria root extract and its medical use in antitumor. Background technique [0002] Tengli root is the root of Actinidia chinensis Planch and A. arguta Planch, which are bitter and cold in nature and have the effect of clearing heat, detoxifying and promoting blood circulation. In recent years, it has been found that the plants of this genus have anti-cancer and blood-lipid-lowering activities. Folks use them to treat gastric cancer, nasopharyngeal cancer, and breast cancer, and have achieved certain curative effects, especially for digestive tract cancer. [0003] In recent years, studies on the antitumor effects of vine root extracts have shown that vine root contains a variety of biologically active components. People such as Wei Jinyu use the Chinese and Vietnamese kiwifruit aerial parts and root extracts to inhibit the growth of the liver cancer cell line BEL-7404 (W...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P35/00
Inventor 张虹向俊峰管烨陈旭征唐亚林
Owner INST OF CHEM CHINESE ACAD OF SCI
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