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Basic catalase high-yield strain and basic catalase prepared by fermentation method using same

A catalase and fermentation technology, applied in the field of bioengineering, can solve the problems of not reaching strong alkalinity, low catalase enzyme activity, not reaching industrialization, etc., improving the environment and product quality, changing dyeing and finishing The effect of the treatment process

Inactive Publication Date: 2007-05-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, most of the products have not yet reached the requirements of strong alkalinity (pH>10) in the textile industry, and the catalase activity produced by Bacillus subtilis in domestic and foreign literature is very low, far from reaching the requirements of industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Scrape the two ring strains from the slope and place them in a 250ml Erlenmeyer flask containing 70ml seed culture medium for seed culture. The conditions are: pH 7.5, culture time 12 hours, temperature 37°C, and shaker rotation speed 200 rpm. Then 6% of the inoculum was inserted into a 250ml Erlenmeyer flask containing 50ml of fermentation medium for fermentation culture. The conditions are: pH 7.5, culture time 35 hours, temperature 37°C, shaker rotation speed 200rPm, and then the fermentation broth with enzyme activity 3200U / ml can be obtained.

Embodiment 2

[0020] Scrape the two ring strains from the slope and place them in a 250ml Erlenmeyer flask containing 70ml seed culture medium for seed culture. The conditions are: pH 7.5, culture time 16 hours, temperature 40°C, and shaker rotation speed 200 rpm. Then, 8% of the inoculum was inserted into a 250ml Erlenmeyer flask containing 50ml of fermentation medium for fermentation culture. The conditions are: pH 7.5, culture time 24 hours, temperature 40°C, and shaker rotation speed 200 rpm. A fermentation broth with an enzyme activity of 2800U / ml can be obtained.

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PUM

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Abstract

A bacterium with high yield of alkaline hydrogen peroxidase and method for producing hydrogen peroxidase through fermenting the bacterium belongs to the field of bioengineering technique. The bacterium of the invention is Bacillus subtilis (CCTCC NO:M206062) screened from effluent water of textile mill workshop through air bubble generation conditions by dropping 5% H2O2 on the plate. Alkaline hydrogen peroxidase with 3200u / ml enzyme activity is obtained by fermenting the bacterium through shaking flasks for deep liquid fermentation. The alkaline hydrogen peroxidase can be used for treating texture dying.

Description

Technical field [0001] A high-producing alkaline catalase bacteria and the fermentation method for producing alkaline catalase by using the strain, in particular a Bacillus strain with high alkaline catalase production, belong to the technical field of biological engineering. Background technique [0002] The textile industry is a major industry in many developing countries, and it is also my country's traditional industry and pillar industry. The textile industry is an industry that produces very serious pollution. Especially in the printing and dyeing process, traditional processes consume a lot of water and chemicals, which not only consume resources, but also cause environmental pollution and disrupt ecological balance. This is essential to the implementation of sustainable development strategies. Extremely unfavorable. To fundamentally change the traditional extensive economic growth mode of high consumption and high pollution in the textile industry, we must vigorously prom...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/08D06M16/00C12R1/125D06M101/04
Inventor 陈坚赵志军华兆哲堵国成
Owner JIANGNAN UNIV
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