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Basic catalase high-yield strain and basic catalase prepared by fermentation method using same

A technology of catalase and fermentation method, which is applied in the field of bioengineering, can solve the problems of not reaching strong alkalinity, low catalase activity, and not reaching industrialization, so as to improve the environment and product quality, and change dyeing and finishing The effect of the treatment process

Inactive Publication Date: 2008-10-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the products have not yet reached the requirements of strong alkalinity (pH>10) in the textile industry, and the catalase activity produced by Bacillus subtilis in domestic and foreign literature is very low, far from reaching the requirements of industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Scrape the two-ring strains from the inclined surface and place them in a 250ml Erlenmeyer flask equipped with 70ml seed culture medium for seed culture. The conditions are: pH 7.5, incubation time 12 hours, temperature 37° C., shaker rotation speed 200 rpm. Then insert 6% of the inoculum into a 250ml conical flask with 50ml fermentation medium for fermentation. The conditions are: pH 7.5, incubation time 35 hours, temperature 37°C, shaker rotation speed 200rPm, and a fermentation broth with an enzyme activity of 3200U / ml can be obtained.

Embodiment 2

[0020] Scrape the two-ring strains from the inclined surface and place them in a 250ml Erlenmeyer flask equipped with 70ml seed culture medium for seed culture. The conditions are: pH 7.5, incubation time 16 hours, temperature 40° C., shaker rotation speed 200 rpm. Then insert 8% inoculum into a 250ml conical flask equipped with 50ml fermentation medium to carry out fermentation culture. The conditions are: pH 7.5, incubation time 24 hours, temperature 40° C., shaker rotation speed 200 rpm. A fermentation broth with an enzyme activity of 2800U / ml can be obtained.

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PUM

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Abstract

A bacterium with high yield of alkaline hydrogen peroxidase and method for producing hydrogen peroxidase through fermenting the bacterium belongs to the field of bioengineering technique. The bacterium of the invention is Bacillus subtilis (CCTCC NO:M206062) screened from effluent water of textile mill workshop through air bubble generation conditions by dropping 5% H2O2 on the plate. Alkaline hydrogen peroxidase with 3200u / ml enzyme activity is obtained by fermenting the bacterium through shaking flasks for deep liquid fermentation. The alkaline hydrogen peroxidase can be used for treating texture dying.

Description

technical field [0001] The invention relates to a high-yielding alkaline catalase bacterium and the production of alkaline catalase through the fermentation method of the bacterial strain, in particular to a high-yield alkaline catalase-producing bacillus, which belongs to the technical field of bioengineering. Background technique [0002] The textile industry is a major industry in many developing countries, and it is also a traditional industry and a pillar industry in our country. The textile industry is a very polluting industry, especially in the printing and dyeing process. The traditional process consumes a lot of water and chemicals, which not only consumes resources, but also causes environmental pollution and destroys the ecological balance. This is very important for the implementation of sustainable development strategies. extremely unfavorable. In order to fundamentally change the traditional extensive economic growth mode of high consumption and high pollutio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/08D06M16/00C12R1/125D06M101/04
Inventor 陈坚赵志军华兆哲堵国成
Owner JIANGNAN UNIV
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