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Electrokinetic chromatography for quantitative analysis of capillary micelle of desmosin and isodesmosine and reagent set

A technique of electrokinetic chromatography and isodesmosin, which is applied in the field of quantitative analysis of desmosins and isodesmosins in biological samples, can solve the problems of unsatisfactory accuracy and ultraviolet detection sensitivity, and the incomplete baseline separation of DES and IDES. Automation and high throughput, efficient separation and automatic operation, relatively small error effect

Inactive Publication Date: 2007-05-16
SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI
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Problems solved by technology

However, this method is still interfered by the complex matrix in biological samples when performing separation and detection, and DES and IDES are not completely separated by baseline, so its accuracy and UV detection sensitivity are not very ideal

Method used

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  • Electrokinetic chromatography for quantitative analysis of capillary micelle of desmosin and isodesmosine and reagent set
  • Electrokinetic chromatography for quantitative analysis of capillary micelle of desmosin and isodesmosine and reagent set
  • Electrokinetic chromatography for quantitative analysis of capillary micelle of desmosin and isodesmosine and reagent set

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Embodiment Construction

[0031] The following examples will help to understand the present invention, but do not limit the content of the present invention.

[0032] Example With the adventitia and media tissue of blood vessels as a sample, the concentration of desmosin and isodesmosin in the sample was determined by capillary micellar electrokinetic chromatography containing mixed micelles composed of PAPS and Brij35; Repeatability, recovery rate, etc.

[0033] 1. Treatment of the capillary: first pretreat the capillary wall with 0.1mol / L sodium hydroxide for 5 minutes, then rinse with water for 5 minutes, then fill the micelle solution into the capillary column for 2 minutes under a pressure of 20 psi, and the column temperature is 25 ℃, pending sample separation and detection.

[0034] 2. Optimization of the buffer: the mixed micelle solution used in the present invention is made up of two kinds of surfactants, PAPS and Brij35. We first investigated the impact of the pH value in the buffer and the...

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Abstract

This invention relates to one quantitative analysis DES and IDES capillary glue beam electrical spectrum method and special agent case, wherein, its buffer liquid comprises the following parts: surface active agent-alkyl sulfonate, wherein, the alkyl is of dodecyl to octadecyl; it recommends 3-(N,N- dimethylcetyl)- alkyl sulfonate (PAPS) and non ion surface active agent alkylpolyglycol ether; the said alkyl is of dodecyl and octadecyl; it recommends carbowax dodecyl to form mix blue beam and background electrolyte water solution.

Description

technical field [0001] The invention relates to a method for quantitative analysis of desmosin and isodesmosin in biological samples. The method can use a special kit, and in particular relates to the establishment of capillary micellar electrokinetic chromatography for quantitative analysis of desmosin and isodesmosin in biological samples and its application. Background technique [0002] Since the 1950s, people began to study the physiological function of elastin. It has been found that as an important structural protein, the change of elastin content has a very important relationship with various diseases in organisms. For example, the content of elastin in the lung plays a very important role in the diagnosis and pathological research of obstructive emphysema, chronic bronchitis and other diseases. In recent years, arterial elastin has been found to be associated with many vascular diseases. It is already known that changes in elastin are associated with various dise...

Claims

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Application Information

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IPC IPC(8): G01N30/02B01D15/00B01J20/281
Inventor 康经武黄靖
Owner SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI
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