Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Use of argyi leaf polysaccharide extract

A technology of polysaccharide extraction and precipitation, which is applied in the field of polysaccharide extract from leaves of Artemisia argyi, and can solve the problems of unreported use of polysaccharide extract from leaves of Artemisia argyi

Inactive Publication Date: 2007-05-16
EAST CHINA UNIV OF SCI & TECH
View PDF0 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the use of Artemisia argyi polysaccharide extract has not been reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of argyi leaf polysaccharide extract
  • Use of argyi leaf polysaccharide extract
  • Use of argyi leaf polysaccharide extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 2.0kg of Artemisia argyi leaves, decoct and extract twice, each time for 1 hour, add 10 times the amount of water each time, and combine the two filtrates. The filtrate was concentrated to 500 mL at 50°C under reduced pressure, and ethanol was added to make the concentration 65v / v%. After standing for 12 hours, it was centrifuged for 20 minutes, the supernatant was discarded, and the precipitate was dried under reduced pressure at 50°C. Mix the dried precipitate with 400mL of deionized water, then add 200mL of 10wt% trichloroacetic acid solution, stir well, leave it for 4 hours, centrifuge for 20 minutes, discard the precipitate, and add ethanol to the clear liquid to make the concentration 75v / v%, ethanol precipitation (standing) for 24 hours and then centrifuged to obtain the precipitate, which was washed with absolute ethanol, acetone, and ether in sequence, and then vacuum-dried at 50°C to obtain 24g of Artemisia argyi polysaccharide extract.

Embodiment 2

[0020] Take 2.0kg of Artemisia argyi leaves, decoct and extract twice, each time for 1 hour, add 10 times the amount of water each time, and combine the two filtrates. The filtrate was concentrated under reduced pressure at 50°C to 500mL, and ethanol was added to make the concentration 80v / v%, after standing for 12 hours, centrifuged for 20 minutes, the supernatant was discarded, and the precipitate was dried under reduced pressure at 50°C. The dried precipitate was mixed with 400 mL of deionized water, and deproteinized by the Sevag method for a total of 7 treatments. Add ethanol to the supernatant obtained after deproteinization by the Sevag method to make the concentration 75v / v%. After alcohol precipitation (standstill) for 24 hours, it is centrifuged to obtain the precipitate, and the resulting precipitate is washed with absolute ethanol and acetone successively. , and ether, and then vacuum-dried at 50° C. to obtain 38 g of Artemisia argyi polysaccharide extract.

[002...

Embodiment 3

[0023] The inhibitory effect of intravenous injection of Artemisia argyi polysaccharide extract (abbreviation: AYTP) extracted by the extraction method described in Example 1 on mouse transplanted tumor Heps:

[0024] Take 40 ICR mice (18-22g, half male and half female; provided by the Shanghai Experimental Animal Center of the Chinese Academy of Sciences), inoculate Heps solid type according to the transplanted tumor research method, weigh the mice 24 hours after inoculation, and divide them into 4 groups randomly. There were 10 rats in each group, half male and half male, and the blank control group and the cyclophosphamide (CTX, 30 mg / kg) group were respectively yin and yang control groups. AYTP group set up high and low dose groups (100, 50mg / kg). 24 hours after inoculation, intravenous injection was administered, once every other day, and a total of 4 administrations were administered. On the second day after drug withdrawal, the mice were weighed, and the tumor-bearing m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of polysaccharide extract of Artemisia argyi, which inhibits mouse transplanting Heps tumor and Eac tumor to improve the activity of mouse NK cell. The extract possesses obvious reinforced immune function, which is dependent on dose.

Description

technical field [0001] The present invention relates to the use of a leaf extract of mugwort, in particular to the use of a leaf polysaccharide extract. Background technique [0002] Artemisia argyi (scientific name: Artemisia argyi), Compositae, perennial herb. Its leaves (Ai Ye) are used as medicine, and the main functions are: dispelling cold and relieving pain, warming the meridians to stop bleeding. [0003] Artemisia argyi contains components such as volatile oil, adenine, choline, tannins, flavonoids, sterols, terpenoids, polysaccharides and trace elements. Seo et al extracted flavonoids from Artemisia argyi and found that it has anti-tumor activity (Planta Med, 2003, 69, 3; J.ofEthno.2005, 3, 26; Jour.OfAgri.And Food Chem.2000, 42, 8); Lee and Ha et al found that the water extract and methanol extract of Folium Artemisiae Argyi can inhibit the proliferation of certain tumor cells (Jour.OfBiochem.And Mole.Biol., 1999, 6, 23; Natural Product Sciences, 1997 , 3, 1). ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00A61K31/715A61P37/02
Inventor 蓝闽波何正有郑颖袁慧慧刘建文翟大宇魏冬
Owner EAST CHINA UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com