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Process for extracting vegetable seed protein and calcium phytate from vegetable seed cake

A technology of rapeseed protein and rapeseed cake, applied to the protein composition of vegetable seeds, peptide preparation methods, chemical instruments and methods, etc., can solve the problem of high calcium and magnesium content in calcium phytate and low protein utilization value , affecting product quality and other issues, to achieve high food safety, expand raw material sources, and low cost

Inactive Publication Date: 2007-05-16
HEFEI UNIV OF TECH
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Problems solved by technology

This traditional manufacturing method has the following shortcoming: (1) sulfuric acid is a strong oxidizing agent, which will produce violent oxidation reaction when meeting air and carbon dioxide, thereby reducing the acid value and affecting the output; (2) need to use a large amount of lime, The calcium and magnesium content in calcium phytate is too large, which affects the product quality
In the past, the preparation of rapeseed protein and calcium phytate was only separated. Since the content of phytic acid in rapeseed cake is very low, the protein with high content will be wasted by separate extraction and the utilization value is not high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] (1) Extraction of rapeseed protein:

[0035] ①Alkaline solution Weigh 100g of crushed rapeseed cake, add sodium hydroxide solution adjusted to pH 12 according to the solid-liquid ratio of 1:10, and stir at 50°C (stirring speed 120-180r / min) for leaching, each time for 20min , A total of 4 extractions (same pH). Combine the extracts after four times of coarse filtration with double-layer gauze and centrifuge at 3000r / min for 10min, separate the supernatant, and carry out vacuum filtration. The lower layer is cake residue, which is used for subsequent extraction of calcium phytate.

[0036] ②Acid precipitation to adjust the pH of the above filtrate to 4.5, let it stand for 2 hours, separate the supernatant, remove the lower precipitate and centrifuge at 3000r / min for 10min, combine the supernatant, wash the precipitate with pH4.5 hydrochloric acid aqueous solution and then centrifuge for 10min, the precipitate is Rapeseed crude protein.

[0037] ③ Freeze-drying The cru...

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PUM

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Abstract

The invention discloses an extracting method of rape protein and phytin from rape refuse, which is characterized by the following: adopting rape refuse as raw material; immersing the protein through sodium hydroxide solution; separating; sucking to obtain residual on the bottom and protein solution; adjusting the isoelectric point of protein solution through alcaine; freezing protein sediment; drying; hyperfiltering the non-sediment protein; spraying; drying; immersing residual through alcaine; neutralizing secondarily; sediment; refining to obtain phytin.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and relates to a comprehensive extraction process of protein and calcium phytate in rapeseed cake, so as to realize the comprehensive utilization of rapeseed processing by-products. Background technique [0002] The output of rapeseed in my country ranks first in the world, and the rapeseed cake produced after oiling from rapeseed reaches 6-7 million tons every year. Rapeseed meal contains a variety of components with high utilization value, such as rapeseed protein, phytic acid (phytin), rapeseed polyphenols, rapeseed polysaccharides, etc. However, the use of rapeseed meal in my country is quite limited at present, and it is only used as fertilizer or added in a small proportion as feed for ruminants and freshwater fish, resulting in a great waste of rapeseed meal resources. In this study, rapeseed protein and calcium phytate were separated and extracted from rapeseed cake in order...

Claims

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Application Information

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IPC IPC(8): C07F9/09A23J1/14C07K2/00C07K1/30
Inventor 姜绍通邵平潘丽军罗水忠蒋连平章炳谊
Owner HEFEI UNIV OF TECH
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