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Method for detecting tubercle bacillus antigen in body fluid

A tuberculosis and antigen technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of non-pulmonary tuberculosis, false positive of BCG, slow growth of tuberculosis, etc.

Inactive Publication Date: 2007-03-28
FORMOSA BIOMEDICAL TECH CORP
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Problems solved by technology

[0005] 2. Chest X-ray (Chest x-ray): Chest X-ray examination is very important for the diagnosis of pulmonary tuberculosis. In addition to judging whether or not infected with pulmonary tuberculosis, this method can also help patients with positive skin tests to do more. Further confirmatory, but non-diagnostic for non-pulmonary tuberculosis
[0007] 4. Bacterial culture (Culture): In order to correctly diagnose tuberculosis, the most accurate method is still bacterial culture, and the drug resistance test can be done after bacterial culture to provide a basis for doctors to administer medicines. However, the growth of tuberculosis is very slow, and the laboratory It takes 4 to 6 weeks for diagnosis, and about 2 to 3 weeks for drug resistance test, which is a very time-consuming diagnostic method
[0008] 5. Polymerase Chain Reaction (Polymerase Chain Reaction, PCR): Polymerase Chain Reaction is one of the diagnostic tools actively developed at present. It is more sensitive than other methods for diagnosing early tuberculosis infection, so some hospitals have also begun to use this method It is a method for diagnosing tuberculosis, but the cost of this method is relatively high, and operators need to be trained, so this method is still more suitable for application in research and development
[0009] 6. Serology test (Serology test): In the past ten years, many research and development teams have been developing serum tests for the diagnosis of tuberculosis, but found that this method cannot distinguish between active tuberculosis patients and previously infected tuberculosis patients; and for people who have been vaccinated with BCG prone to false positives
[0010] About one-third of the world's population is infected by tuberculosis, so how to diagnose tuberculosis and related diseases early is very important, especially how to isolate tuberculosis patients or patients infected with multidrug-resistant tuberculosis is the key to control tuberculosis However, due to the incomplete methods for clinically diagnosing tuberculosis at present, the correct rate of diagnosis is low
[0011] Therefore, in order to improve the shortcoming of the low correct rate of current tuberculosis diagnostic methods, the inventors selected the protein secreted by Mycobacterium tuberculosis as the specific antigen, and produced its polyclonal and monoclonal antibodies

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  • Method for detecting tubercle bacillus antigen in body fluid
  • Method for detecting tubercle bacillus antigen in body fluid
  • Method for detecting tubercle bacillus antigen in body fluid

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Embodiment Construction

[0036] The present invention is a method for detecting Mycobacterium tuberculosis antigen in body fluid, which mainly includes the following aspects:

[0037] (a) Any antigen-antibody binding reaction method to detect specific antigens secreted by Mycobacterium tuberculosis in various body fluids;

[0038] (b) Monoclonal and polyclonal antibodies against tuberculosis-specific antigens;

[0039] (c); reagent kits for specific antigens secreted by Mycobacterium tuberculosis, and

[0040] (d) Detection of Mycobacterium tuberculosis antigen using the above-mentioned antibody or kit.

[0041] Wherein, the reagent kit includes:

[0042] (a) The antibody in the stationary phase has the ability to recognize the antigens or synthetic polypeptides translated by the RD1, RD2, and RD3 gene groups in Mycobacterium tuberculosis;

[0043] (b) The marker attached to the secondary antibody may include enzyme, fluorescent, luminescent, radioactive substance markers; and;

[0044] (c) Recomb...

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Abstract

One mycobacterium tuberculosis antigen detective method in tuberculosis bacilli solution, including the following steps: (a) Choice and prepare TB-specific antigen that can be detect by the antigen-antibody binding reactions in various body fluids; (b) Prepare of confrontation and strains of TB-specific antigen monoclonal antibody; (C) Prepare TB detection reagent kit of specific antigen secreted; (d) Use the above-mentioned antibody and antigen detection kit for tuberculosis. Take these steps and reach high rate of the test and correct testing purposes.

Description

technical field [0001] The invention relates to a method for detecting Mycobacterium tuberculosis antigen in body fluid, in particular to a detection method which can directly detect specific antigen secreted by Mycobacterium tuberculosis in body fluid. Background technique [0002] The infection route of tuberculosis is mainly through droplets in the air, and the main pathogenic bacteria causing tuberculosis is Mycobacterium tuberculosis. According to the report of the World Health Organization, there are currently more than 8 million new cases of tuberculosis every year. And about 3 million people die from tuberculosis every year, and about 33% of the world's population is latent tuberculosis patients, so how to detect and diagnose tuberculosis patients quickly and correctly is very important. [0003] Currently, the most common methods for diagnosing tuberculosis include the following: [0004] 1. Skin test (Skin test): This is the most common preliminary diagnosis metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/569
Inventor 詹尔昌杨明荧
Owner FORMOSA BIOMEDICAL TECH CORP
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