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Method of consolidating heavy metal ion by microorganism

A technology of heavy metal ions and microorganisms, applied in the restoration of contaminated soil, bacteria, flocculation/sedimentation water/sewage treatment, etc., can solve the problems of poor effect, high cost, secondary pollution, etc., and achieve high practical value and economical Convenience, the effect of ensuring the safety of life

Inactive Publication Date: 2007-03-07
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Physical and chemical methods are often expensive, and the effect is not good, it is easy to cause secondary pollution, and it is not suitable for large-scale, low-concentration heavy metal pollution

Method used

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  • Method of consolidating heavy metal ion by microorganism
  • Method of consolidating heavy metal ion by microorganism
  • Method of consolidating heavy metal ion by microorganism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The method for microorganisms to fix heavy metal ions, and the expansion of bacterial strains: Inoculate the strain Bacillus pasteurii (Bacillus pasteurii) into LB medium with urea as the substrate, shake and cultivate in a shaking incubator at 25-37°C, and cultivate 12-24 hours; adding the bacterial suspension obtained above into the soil or solution containing heavy metal ions to produce microorganism-heavy metal composite flocs, and further generate water-insoluble carbonates. When the bacterial suspension is added to the soil or the solution is mixed with the calcium chloride solution, the Ca 2+ The molar concentration is 0.5 to 2 times the molar concentration of heavy metal ions in soil or solution.

Embodiment 2

[0035] Inoculate the strain of Bacillus pasteurianus on the LB medium in the Erlenmeyer flask. The medium composition is shown in Table 1. Shake culture in a constant temperature shaking incubator at 25-37°C, take it out after 12-24 hours of culture, and store it at room temperature. Add 1mol / L CdCl 2 , precipitate rapidly, filter it, dry it, and measure the precipitate (as shown in Figure 1). It can be seen that in the microbial environment, heavy metal ions are consolidated into carbonates to achieve the purpose of mineralizing heavy metals.

[0036] Nutrients

Embodiment 3

[0038] Inoculate the strain of Bacillus pasteurianus on the LB medium in the Erlenmeyer flask, shake and cultivate in a constant temperature shaking incubator at 25-37°C, take it out after culturing for 12-24 hours, and add 0.5mol / L CaCl at room temperature 2 , 1mol / LCdCl 2 Solution, precipitated rapidly, was filtered and dried, and was qualitatively analyzed by XRD as co-crystal substance A being Ca 0.67 Cd 0.33 CO 3 , B is CaCO 3 , C is CdCO 3 Part of the heavy metal Cd and Ca co-crystallized in the form of carbonate, the particle morphology is shown in Figure 3 and Figure 4, mostly spherical.

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Abstract

The invention discloses a fixing method of heavy ion through microbe, which comprises the following steps: inoculating Bacillus pasteurii in the LB culture medium based on aquacare as base; vibrating under 25-37 deg.c; culturing for 12-24h; adding bacteria suspension in the soil or solution with heavy metal ion as well as Ca2+ solution to produce composite floccule body of microbe-heavy metal; generating insoluble carbonate.

Description

technical field [0001] The invention relates to a method for microorganisms to consolidate heavy metal ions, more specifically, the invention relates to a method for microorganisms to consolidate heavy metal ions through mineralization. Background technique [0002] There are physical and chemical methods and biological methods to control heavy metal ions. Physicochemical methods are often expensive, and the effect is not good, it is easy to cause secondary pollution, and it is not suitable for large-scale, low-concentration heavy metal pollution. The microbial treatment of heavy metal pollution in biological methods is an emerging treatment method, which is an environmentally friendly and green treatment method with low cost. [0003] Bioremediation is a method of using natural or artificially modified organisms as a whole or components to treat environmental pollutants. It has the characteristics of low investment, high efficiency, and in-situ treatment of low-concentrati...

Claims

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Application Information

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IPC IPC(8): C02F1/52B09C1/10C12N1/21
Inventor 钱春香王瑞兴成亮吴淼
Owner SOUTHEAST UNIV
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