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Method of detecting proliferation function of plrphyra yezoensis crude polysaccharide to rat testicle sertoli cell

A technology of testicular Sertoli cells and Porphyra zebra, which is applied in the field of detecting the effect of Porphyra zebra polysaccharides on the proliferation of rat testicular Sertoli cells, to achieve the effect of expanding application prospects and increasing application scope

Inactive Publication Date: 2007-02-28
SHANGHAI FISHERIES UNIV
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Problems solved by technology

[0003] At present, there is no relevant report on the effect of laver polysaccharides on Sertoli cells in rat testes. Our study found that the proliferative effect of Porphyra zebra polysaccharides on Sertoli cells in rats may be useful for the joint transplantation of Sertoli cells and other functional cells and Improving sperm quality and the treatment of male infertility has great guiding significance and may open up new avenues

Method used

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  • Method of detecting proliferation function of plrphyra yezoensis crude polysaccharide to rat testicle sertoli cell
  • Method of detecting proliferation function of plrphyra yezoensis crude polysaccharide to rat testicle sertoli cell
  • Method of detecting proliferation function of plrphyra yezoensis crude polysaccharide to rat testicle sertoli cell

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Embodiment Construction

[0010] In the following, the method for detecting the effect of the Porphyra zebra polysaccharide on the proliferation of Sertoli cells of rats will be further described in detail in conjunction with the accompanying drawings.

[0011] Method implementation steps of the present invention are as follows:

[0012] (1) Primary culture of rat testicular Sertoli cells:

[0013] 1. A male SD rat was killed by decapitation with a special knife for animals, and the testicular tissue with capsule was removed;

[0014] 2. Use scissors to cut an incision about 1 cm long on the testicular foreskin, and use forceps to gently extrude the testis inside the skin and remove the blood vessels, so that you can get the testicular tissue with most of the blood vessels removed;

[0015] 3. Put the testicular tissue on a sterilized glass plate, absorb a small amount of pre-cooled (stored at 4°C) normal saline (no Ca2+, Mg2+) with a dropper, and rinse the testicular tissue 2 to 3 times;

[0016] 4....

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Abstract

The invention relates the method for checking laver raw sugar to hold cell proliferation action for mouse testis. Now there are no related reports. The invention uses MTT method to check the breeding rate, and the results express that the more the raw sugar, the stronger the breeding action. The invention can hold cell to graft together with other cell and improve the spermium quality.

Description

Technical field: [0001] The invention relates to a method for detecting the proliferative effect of the Porphyra variegata crude polysaccharide on the Sertoli cells of rats. Background technique: [0002] Sertoli cells can secrete immune protection factors, a variety of growth factors and nutritional factors, and have attracted the attention of scholars. Experimental studies have reported that Sertoli cells in the testis undertake the immune immunity function, and co-transplanting Sertoli cells can prolong the survival of allografts. Survival time has extended the functional research of testicular Sertoli cells to the field of organ transplantation, and has shown good clinical application prospects. Some people have compared Sertoli cells with other types of cells such as rat fetal brain dopaminergic neurons and pig pancreatic cells. Combined culture, in order to facilitate the growth, differentiation and proliferation of such cells, and some people will jointly transplant S...

Claims

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Application Information

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IPC IPC(8): C12Q1/04G01N33/48
Inventor 郭婷婷何培民
Owner SHANGHAI FISHERIES UNIV
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