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Embedding protection method for beneficial bacteria of intestinal tract

A technology of intestinal probiotics and protective agents, which is applied in the field of preparation of microbial preparations, can solve the problems of easy inactivation of probiotics, and achieve the effect of prolonging activity and increasing stress resistance

Active Publication Date: 2007-02-21
哈尔滨美华生物技术股份有限公司
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AI Technical Summary

Problems solved by technology

[0010] However, probiotics are easily inactivated before production, storage and entering the human intestinal tract. In order to obtain higher viable bacteria, it is necessary to protect the probiotics

Method used

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Experimental program
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Embodiment Construction

[0022] First, culture Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, and Bifidobacterium bifidum test tube strains. The strain culture medium is 12% skimmed milk powder, inoculated after sterilization and cooling, and cultivated at 37°C for 16 hours, and then the test tubes The strains were cultured in a Erlenmeyer flask liquid medium, cultured at 37°C for 18 hours, and then expanded and cultivated step by step, and cultured in large tanks. After 18 hours of culture, the bacteria sludge was obtained by high-speed centrifugation.

[0023] Then, an embedding protectant was added, and the formulation of the embedding protectant was: 10.0% skimmed milk powder, 2.5% sodium glutamate, 8.0% lactose, 3.5% glycerin, 1.0% polyvinylpyrrolidone, and 1.5% Vc. The embedding protectant was sterilized at 105°C and cooled to 30°C for later use.

[0024] The embedding protectant and bacteria slime are mixed according to the ratio of protectant: bacteria sludge = 1:2, a...

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Abstract

This invention relates to a protection method for embedding intestinal probiotic bacteria. The method comprises: (1) culturing intestinal probiotic bacteria in a fermentation tank in large scale so that the number is 1.5*109 cfu / mL, and centrifuging in a high speed to obtain bacterial mud; (2) adding special embed-protecting agent so that the ratio of the embed-protecting agent to bacterial mud is 0.1 : (0.1-10.0), and freeze-drying to obtain active microbe strain containing inner-embedding layer as Pa; (3) preparing middle-layer embedding agent as B1; (4) mixing Pa and B1 at a ratio of 1 : (0.1-3) to obtain a middle-emdedding layer; (5) subjecting the active bacteria containing middle-embedding layer to outer-layer embedding, and naturally drying to obtain active bacteria containing three embedding layers. The method can make intestinal probiotic bacteria target-propagate after safely reaching small intestine and large intestine.

Description

(1) Technical field [0001] The invention relates to a method for preparing a microbial preparation, in particular to a method for protecting an intestinal microecological preparation. (2) Background technology [0002] Prebiotics or probiotics refer to the beneficial physiological effects of certain microbial preparations or fermented products on the host by regulating the host's mucosal and systemic immune functions and improving intestinal nutrition and flora balance. Although some lactobacilli and bifidobacteria cannot reach the intestinal tract in a viable state and colonize there, their cell wall components can still play anti-tumor and immune-regulating effects, for example, heat-inactivated Lb. Health is promoting. In 1907, Metchmikoff believed that regular consumption of fermented milk was beneficial to human health based on his research on Bulgarian bacillus, which was very similar to Lactobaillus delbruekii subsp.bulgaricus used in modern yogurt growth. Lactobaci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/04C12R1/46C12R1/225C12R1/23
Inventor 肖雯娟
Owner 哈尔滨美华生物技术股份有限公司
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