Method for mass expressing external protein using domestic silk core protein heavy chain promoter

Abstract

The invention relates to a method useing cultivated silkworm fibroin heavy chain promoter to express extraneous sources protein. It can increase extraneous sources protein content to 16% which is greatly higher by using light chain protein and fhx promoter. This can broaden cultivated silkworm application field. And it can be used to easily gain transgene silkworm concoon to get purpose extraneous sources protein by feeding purpose transgene silkworm largely.

Description

Technical field [0001] The present invention relates to the field of biotechnology, and in particular to a method for expressing a large amount of exogenous protein using the promoter of silk fibroin heavy chain. Background technique [0002] Bombyx mori is an important economic and model organism. It has made great contributions to the development of my country's socialist economy and is making further contributions to the sustainable development of China's economy. The first completion of the silkworm genome framework led by the China Silkworm Genome Project research team not only established the international status of my country's silkworm functional genome research, but also provided new opportunities for the development of the silk industry and entomology. The main work in the functional genomics period includes: identification and cloning of major functional genes, elucidation of the molecular mechanisms of important biological traits, and ultimately the realization o...

AI Technical Summary

Problems solved by technology

The recently reported production of foreign proteins in the transgenic silk glands of silkworms all use the light chain protein and the fhx promoter. These silkworm expression systems cannot express foreign proteins very effectively because these two components are originally the two components of fibroin. non-essential ingredient

The development of the heavy chain protein promoter, the main component of silkworm fibroin, will help to greatly increase the proportion of exogenous recombinant protein in the transgenic silkworm cocoons, and in silkworm embryos using the transposon piggyBac derived from Lepidoptera In the research report of microinjection transgenic technology, there is no report about the method of using the silkworm fibroin heavy chain promoter to express a large amount of foreign protein