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Production of anti-CD20 intrinsic antibody and its use

A chimeric antibody, a new type of technology, applied in the direction of antibodies, anti-animal/human immunoglobulins, fusion cells, etc., to achieve moderate molecular weight, good clinical application prospects, and good stability.

Inactive Publication Date: 2007-01-17
BEIJING MABWORKS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large number of experiments have shown that the epitopes recognized by anti-CD20 antibodies are diverse [5]

Method used

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  • Production of anti-CD20 intrinsic antibody and its use
  • Production of anti-CD20 intrinsic antibody and its use
  • Production of anti-CD20 intrinsic antibody and its use

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Experimental program
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Embodiment Construction

[0017] The process of constructing the TGS antibody based on the anti-CD20 monoclonal antibody (1-28) gene is described in detail below, and the activity of the novel antibody is verified through biological experiments. It must be pointed out that this method is not limited to the 1-28 antibody gene preserved in this laboratory, other CD20 antibody genes and other antibody genes that are expected to reduce murine origin can be applied to prepare new antibodies with this structure.

[0018] Design and functional characterization of TGS

[0019] 1. Materials

[0020] The hybridoma cell 1-28 (IgM) secreting anti-CD20 antibody was stored in Beijing Tianguangshi Biotechnology Co., Ltd.

[0021] Daudi, Raji, and 293T cells were subcultured with 10% FCS RPMI-1640 and 10% FCS DMEM (Gibco BRL Company) respectively.

[0022] AMV reverse transcription kit and pGEM  -T Easy was purchased from Promega Company.

[0023] The eukaryotic expression vectors pCMV-VH and pCMV-VL used to cons...

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PUM

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Abstract

Production of CD20 chimeric antibody and its use are disclosed. The process is based on preparation of anti-human CD20 single-cloning antibody hybridoma cell 1-28 and its antibody gene. It has cognitive epitope characteristic and mediated killer target cell functions.

Description

technical field [0001] The invention relates to a novel monoclonal antibody specifically targeting CD20 (named 1-28), which has the functions of recognizing CD20 molecules, inhibiting the growth of CD20 positive cells, and specifically killing target cells through CDC; Good prospects for clinical application. [0002] The present invention relates to a novel humanized mouse monoclonal antibody method: the variable region of the antibody is ScFv; the constant region is the Fc segment of IgG, that is, the CH2 domain and CH3 domain of the antibody; the variable region and the constant region are connected through the hinge region of the antibody; Under natural conditions, the cysteine ​​in the hinge region forms a disulfide bond and dimerizes into a bivalent structure. The anti-CD20 humanized antibody TGS achieved by this method greatly reduces the murine origin of mAb 1-28, while maintaining good antigen recognition activity and CDC function, and improves the clinical applicati...

Claims

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Application Information

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IPC IPC(8): C12N5/16C07K16/18A61K39/395A61P35/00
Inventor 沈倍奋冯建男王玉刚黄英谷欣黎燕
Owner BEIJING MABWORKS BIOTECH
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