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A technology of zoysia and cold resistance, which is applied in the field of improving plant stress resistance, and can solve the problems of difficult breeding methods and other problems of zoysia breeding
Inactive Publication Date: 2006-12-13
BEIJING AGRO BIOTECH RES CENT +1
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[0004] Practice has proved that the traditional Zoysia breeding method is difficult to achieve the purpose of breeding in a short period
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Embodiment 1
[0056] Embodiment 1, the cultivation of the zoysia grass that cold resistance improves
[0057] Taking Zoysia sinica (Zoysia sinica Hance) as example, the method for improving the cold resistance of Zoysia sinica Hance of the present invention is described in detail, and concrete method comprises the following steps:
[0058] 1. Acquisition of embryogenic callus from Zoysia sinensis
[0059] Callus induction medium: Add 2.0mg / L2, 4-D, 0.05-0.2mg / L6-BA, 30g / L sucrose, 7g / L agar, pH 5.8 on the basis of MS basic medium.
[0060] Subculture medium: on the basis of MS basic medium, add 1mg / L2, 4-D, 0.2-0.5mg / L6-BA, 30g / L sucrose, 7g / L agar, pH 5.8.
[0061]Proliferation and maintenance culture medium: Add 1mg / L2, 4-D, 0.5mg / L6-BA, 30g / L sucrose, 7g / L agar, pH 5.8 on the basis of MS basic medium.
[0062] The callus differentiation medium is based on the MS basic medium with the addition of 1mg / L6-BA, 0.2mg / Lα-naphthaleneacetic acid, 30g / L sucrose, 7g / L agar, pH 5.8.
[0063] The...
Embodiment 2
[0100] Example 2, Detection of Cold Resistance of CBF1 / DREB1b Transgenic Zoysia sinensis
[0102] The CBF1 / DREB1b transgenic Zoysia sinensis strains Z20, Z19, Z14 obtained in Example 1 and the wild-type control plants were planted and multiplied separately, and after 2 months of recovery, the healthy and consistent plants were placed at 7°C, Light (120umol m -2 the s -1 ) for 2 weeks in an artificial climate chamber for 24 hours, record the rate of yellow leaves (yellow leaves / total leaves), and then resume growth at room temperature (25-30° C.) for 2 weeks to observe the growth of the plants.
[0103] Results After 1 week of low temperature treatment at 7°C, the tip or part of the leaves of the control line began to turn yellow, while the three transgenic lines did not have this phenomenon; , Z19 and Z14 transgenic lines had only 9.7%, 1.5% and 3.2% rolled leaves, respectively. After 2 weeks ...
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Abstract
The invention discloses a method for improving the winter resistance of Japanese lawngrass. This method includes converting transcription activation factor CBF1/DREB1b of quasi-mustard induction gene into Japanese lawngrass embryonal callus to improve the winter resistance of Japanese lawngrass, which can include the following steps: 1) disseminating the Japanese lawngrass embryonal callus with agricillin liquor carrying CBF1/DREB1b; 2) placing the disseminated Japanese lawngrass embryonal callus onto the coculture medium to perform coculture; 3) placing the cocultured embryonal callus onto the screening culture medium for screening; 4) placing the screened embryonal callus onto the differentiated culture medium for differentiation culture; 5) placing the differentiated and germinated young plant onto the rooted culture medium for radication; 6) placing the plant which can produce root onto strong-plant screening culture medium for screening the Japanese lawngrass strong-plant which has improved winter resistance. The invention has important theoretical and operation significance for culturing new species of Japanese lawngrass with improved cold resistance and extended green period and has a wide application foreground.
Description
technical field [0001] The invention relates to a method for improving stress resistance of plants, in particular to a method for improving cold resistance of zoysia grass. Background technique [0002] In order to beautify the city and improve the environment, many cities in northern my country vigorously build lawns, but most of them are cool-season turfgrasses. Although the cool-season turfgrass has a long green period and beautiful color, it consumes a lot of water. It needs to be trimmed frequently because of its rapid growth. It is easy to be infected by diseases and insect pests in summer, and the maintenance cost is high. my country is a developing country with limited economic strength and lack of fresh water resources, especially in the northwest region. Therefore, whether it is urban greening or water and soil conservation projects, we should develop lawns and ground cover plants that are drought-resistant, disease-resistant, extensively managed, and have a long g...
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