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Biosensor and method for preparation thereof

A biosensor and manufacturing method technology, applied in the field of biosensors, can solve problems such as complicated operation, time-consuming, and reduced measurement accuracy, and achieve the effects of eliminating measurement errors, preventing electrodes from rusting, and excellent measurement accuracy

Active Publication Date: 2006-12-06
ARKRAY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is a problem that an error occurs in the oxidation current value obtained by electrochemical reoxidation, thereby reducing the measurement accuracy.
[0004] As a method of avoiding this problem, for example, measurement under a nitrogen atmosphere is conceivable, but this method takes time and is complicated to operate
In addition, there is also a problem that the oxidoreductase that requires oxygen in the enzymatic reaction cannot be used under the condition of complete absence of oxygen, and the applicable range becomes very narrow.

Method used

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  • Biosensor and method for preparation thereof
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  • Biosensor and method for preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0070] An example of the first biosensor manufacturing method of the present invention is as follows figure 1 and figure 2 based on the description. figure 1 (A) to (F) are perspective views showing a series of steps of manufacturing the biosensor. figure 2 for the above figure 1 (F) I-I cross-sectional view of the shown biosensor. figure 1 (A)~(F) and figure 2 with the same symbol in the same position.

[0071] Such as figure 1 (F) and figure 2 As shown, this biosensor 1 is provided with a substrate 11, an electrode system composed of a working electrode 12 having a lead portion 12a and a counter electrode 13 having a lead portion 13a, an insulating layer 14, a layer containing a medium, a layered inorganic compound, and a surfactant. An inorganic gel layer (anti-oxidation layer) 16 , an enzyme reagent layer 17 containing oxidoreductase, a separator 18 having an opening, and a cover 19 having a through hole 20 . Such as figure 1 As shown in (B), one end portion o...

Embodiment 1

[0130] As shown below, making the same as above figure 1 (F) Glucose sensor with the same structure.

[0131] First, a PET substrate (50 mm in length, 6 mm in width, and 250 μm in thickness) is prepared as the insulating substrate 11 of the glucose sensor, and the working electrode 12 and the counter electrode each having a lead portion are formed on one surface by screen printing. 13 carbon electrode system.

[0132] Next, an insulating layer 14 is formed on the above-mentioned electrodes as follows. First, the insulating resin polyester was dissolved in the solvent carbityl acetate so as to have a concentration of 75% by weight to prepare an insulating paste, which was screen-printed on the above-mentioned electrodes. The printing conditions are 300 mesh screen, rubber roller pressure 40kg, and the printing volume is per 1cm 2 The electrode area is 0.002mL. Screen printing is not performed on the detection part 15 and the lead parts 12a and 13a. Then, insulating layer 1...

Embodiment 2

[0143] In this example, the glucose sensor produced in the above-mentioned Example 1 was used to measure the change with time of the response current corresponding to the glucose concentration.

[0144] As a liquid sample, human whole blood was used. First, the whole blood after blood collection was left at 37° C. for about one day, and the glucose concentration was adjusted to 0 mg / 100 mL. Then, glucose was added thereto to prepare samples of various glucose concentrations (approximately 200, 400, and 600 mg / 100 mL). Glucose-free whole blood was adjusted to a glucose concentration of 0 mg / 100 mL. Then, as Example 2, a voltage of 200 mV was applied to the above-mentioned glucose sensor 1 , and then the sample was dropped on the sample supply part 21 , and the time course of the response current 5 seconds after the drop was measured. As Comparative Example 2, the glucose sensor of Comparative Example 1 was measured in the same manner. Each of Examples and Comparative Example...

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Abstract

The present invention provides a biosensor that can prevent a mediator from being affected by oxygen, thereby allowing an analyte in a sample solution to be measured rapidly and easily with high accuracy. The biosensor can be produced by providing a substrate having electrodes, applying a solvent containing a mediator, a surfactant, a buffer, and a layered inorganic compound to surfaces of the electrodes to form an inorganic gel layer for preventing natural oxidation of the mediator, and forming an enzyme reagent layer containing an oxidoreductase on the inorganic gel layer. In this biosensor, due to the inorganic gel layer, the mediator having been reduced by the reaction between an analyte and the oxidoreductase can be measured electrochemically, without being reoxidized by dissolved oxygen or the like.

Description

technical field [0001] The present invention relates to a biosensor for electrochemically measuring an object to be measured in a sample. Background technique [0002] Conventionally, biosensors capable of quantifying a specific object of measurement simply and rapidly without diluting or stirring the sample solution containing the object of measurement have been widely used. This kind of biosensor can be produced as follows (for example, refer to Patent Document 1): for example, on an electrically insulating substrate, an electrode system having an active electrode (also called a measuring electrode) and a counter electrode is formed by screen printing or the like, and A reagent layer containing an oxidoreductase, a mediator (electron transport substance) and the like that react with the above object to be measured is formed on the substrate, thereby producing it. When the sample solution containing the object to be measured is brought into contact with the reagent layer, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327C12Q1/00
CPCC12Q1/004C12Q1/26C12Q1/00G01N33/50
Inventor 山冈秀亮辻朋吾
Owner ARKRAY INC
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