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Salt-resistant gene in wheat

A wheat salt-tolerant gene and gene technology, applied in the field of genetic science, can solve the problems of few reports on wheat salt-tolerant genes

Inactive Publication Date: 2006-10-04
HEBEI NORMAL UNIV
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Problems solved by technology

At present, the research on wheat salt-induced related gene TaSIl is still blank, and there are few reports on wheat salt-tolerant genes in the open literature

Method used

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  • Salt-resistant gene in wheat
  • Salt-resistant gene in wheat
  • Salt-resistant gene in wheat

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Embodiment

[0057] The full length of the wheat salt-tolerant gene provided by the present invention is 462bp, wherein the open reading frame (ORF) contains 459bp and encodes 153 amino acids. By cloning the gene into a prokaryotic expression vector, a prokaryotic expression product with a molecular weight of 16.8KD, that is, the protein encoded by the gene, can be expressed in Escherichia coli.

[0058] Above-mentioned TaSI1 gene and expression product thereof can be obtained by the following steps:

[0059] Using Pu Nong 3665 to cross F 1 The pollen culture, EMS mutagenesis, and repeated screening of salt tolerance yielded wheat salt-tolerant mutant RH8706-49 (salt tolerance index ≥ 1.3) and salt-sensitive mutant H8706-34 (salt tolerance index ≤ 0.5) from the offspring of the same single grain. ), through the following steps:

[0060] ①Using these two materials, after the seeds were sterilized, they were treated with water and 0.4% NaCl and cultured in the dark at 25°C for 6 days, and ...

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Abstract

The invention obtains the specific expression protein of wheat salt tolerant mutant, and finds by mass spectrum identification, Web protein database search and bio-information technique that the protein has high homology with one assumed protein on the 4th rice chromosome. Accordingly, it designs primer, amplifies by PCR to obtain the corresponding wheat salt tolerance gene with 153 amino acids and 462bp length; clones the gene to construct pronucleus expression vector to express out a specific protein with molecular weight as 16.8KD and improve the salt tolerance of escherichia coli obviously. This invention settles foundation for wheat salt tolerance mechanism.

Description

technical field [0001] The invention relates to a wheat salt-tolerant related gene TaSIl (Triticum asetivum SaltInduced 1), which belongs to the technical field of gene science. Background technique [0002] Studies have shown that the most important factor affecting the growth and yield of crops is environmental stress, among which salt stress and drought stress are the most serious. There are about 500 million mu of saline soil in my country, including more than 100 million mu of saline cultivated land and more than 300 million mu of saline wasteland to be reclaimed and utilized. The area of ​​secondary salinized soil is still expanding, causing heavy losses to agricultural production . How to alleviate the impact of salt stress on agricultural production, so that large areas of saline-alkali land, desertified land and abundant salt water resources can be used for agricultural production is an important issue for future agricultural development. Since the mechanism of sal...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82C12N1/21C12Q1/68
Inventor 黄占景郭晓莉葛荣朝赵宝存郭凤沈银柱
Owner HEBEI NORMAL UNIV
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