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Method and equipment for cultivating anaerobic ammonium-oxidizing bacteria

A technique of ammonia oxidizing bacteria and a culture method, which is applied in the field of culture of anaerobic ammonia bacteria, can solve the problems of slow proliferation, long time of culture or domestication, and low yield of bacteria, so as to reduce processing costs and supply costs Effect

Active Publication Date: 2006-09-20
HITACHI LTD
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Problems solved by technology

[0012] However, the above-mentioned microorganisms utilized in wastewater nitrogen removal treatment generally have a slow proliferation rate and a low bacterium yield
Therefore, a large number of bacterial cells of these microorganisms are required in wastewater treatment, so it takes a lot of time to cultivate or acclimatize the microorganisms.

Method used

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Embodiment Construction

[0062] Next, preferred embodiments of the method and apparatus for cultivating anaerobic ammonium oxidizing bacteria of the present invention will be described in detail with reference to the accompanying drawings.

[0063] The inventors of the present invention obtained the following viewpoints during the course of realizing the present invention.

[0064] (1) Examining the doubling time of at least one kind of anammox bacteria containing at least one of the sequences shown in the sequence numbers 1 to 9 of the sequence table in a specific region of the DNA base sequence based on the 16SrRNA gene, the result was not the same as before About 11 days was reported, but a maximum of 1.8 days, in order to cultivate anaerobic ammonia oxidizing bacteria with this doubling time requires a culture method commensurate with this technology. Hereinafter, the anaerobic ammonium oxidizing bacteria containing at least one of the sequences shown in the sequence numbers 1 to 9 of the sequence...

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Abstract

The present invention can feed substrates without waste to generate seed sludge with high bacterial cell concentrations and can start up operation within a short time, in cultivating anaerobic ammonium-oxidizing bacteria with ammonium and nitrite as substrates. Equipment (10) for cultivating anaerobic ammonium-oxidizing bacteria, which cultivates, in a cultivation tank (12), novel anaerobic ammonium-oxidizing bacteria that anaerobically denitrify nitrite and ammonium used as substrates, comprises an ammonium feed device (14) which feeds ammonium at a given concentration into the cultivation tank (12), a nitrite feed device (16) which feeds nitrite at a given concentration into the cultivation tank (12), and a control device (22) which controls a feed rate Y of the substrate.

Description

technical field [0001] The present invention relates to a method and device for cultivating anaerobic ammonium oxidizing bacteria, in particular to a method for removing nitrogen from nitrogen-containing liquids such as waste water, using ammonia and nitrous acid as substrates in nitrogen-containing liquids A method and apparatus for cultivating anaerobic ammonia oxidizing bacteria for efficiently cultivating anaerobic ammonia oxidizing bacteria for anaerobic oxidation treatment. Background technique [0002] Nitrogen components contained in nitrogen-containing liquids such as sewage and industrial wastewater need to be removed before discharging nitrogen-containing liquids out of the system for reasons such as causing eutrophication in water systems such as lakes and swamps, and reducing dissolved oxygen in water systems. nitrogen content. As nitrogen components contained in this nitrogen-containing liquid, ammoniacal nitrogen, nitrite nitrogen, nitrate nitrogen, and organ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/31
CPCC02F2305/06C02F2209/14C02F3/006C02F3/305C02F3/307C12N1/20C02F3/10Y02W10/10
Inventor 井坂和一角野立夫常田聪
Owner HITACHI LTD
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