Degradation bacteria for carbendazim pesticide residue and bacterial agent produced thereby
A pesticide residue degradation and carbendazim technology, which is applied in the field of carbendazim pesticide residue degradation bacteria and the bacterial agents produced, can solve the problems of ecological system hazards, agricultural product exports that affect health, and pesticide residues exceeding the standard, etc., to achieve protection The effect of ecological environment, protection of health, and low cost of production and use
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[0017] Examples of the present invention are described below.
[0018] Activate the original seed of the degrading bacteria DJL-6 of the present invention on a petri dish, measure its degradation performance, and inoculate it on the inclined surface of a test tube for future use. The test tube seed was inoculated in a 1000ml shake flask, and 200ml of LB medium was added to it, and the culture was shaken at a constant temperature to the logarithmic phase, ready to inoculate the seed tank. 500 liters of seed tanks, 400 liters of feeding capacity, the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure damp heat sterilization, after cooling to 33°C, inoculate the above-mentioned cultivated shake flask strains into a 500-liter seed tank according to the inoculum amount of 10%, and cultivate to the logarithmic growth phase, and the s...
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