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Method for diagnosing endometriosis-related disease in womb

A technology for endometriosis and diagnostic method, which is applied in the field of molecular biology diagnosis and can solve problems such as unclear correlation

Inactive Publication Date: 2006-08-23
PERIODOCK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, recent reports have indicated that exposure to TCDD is not associated with endometriosis (Non-Patent Literature 19, 20), resulting in an unclear association between exposure to dioxins and endometriosis

Method used

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  • Method for diagnosing endometriosis-related disease in womb
  • Method for diagnosing endometriosis-related disease in womb
  • Method for diagnosing endometriosis-related disease in womb

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0167] Preparation of polyclonal antibodies

[0168] Antiserum containing anti-HRF antibody was prepared, and anti-HRF antibody was purified from the serum.

[0169] As the sensitizing antigen, the peptide at positions 101-116 in the synthetic human HRF protein (SEQ ID NO: 2) can be selected. The synthesized peptide was combined with HLA as a carrier, and then mixed with KLH (50 µg of KLH was added to 50 µg of peptide). The thus obtained antigen solution was mixed with Freund's complete adjuvant to prepare a solution containing the sensitizing antigen. This solution was subcutaneously injected (5 times) into rabbits (SPF Japanese White Rabbit) with a body weight of 3-4 kg in an amount of 1 ml every 2 weeks.

[0170] Then, after the fifth subcutaneous injection, blood was collected from the rabbit at intervals of one week to prepare serum. It was confirmed that the antibody contained in the obtained serum could specifically recognize the HRF protein, and this was used as an ...

Embodiment 3

[0176] Sandwich EIA

[0177] According to the following method, at least one of the anti-HRF antibodies prepared in Example 2 and known anti-HRF antibodies is selected, and the HRF protein is specifically detected and measured by an appropriate combination of the two anti-HRF antibodies, thereby constituting a sandwich EIA system. The EIA system can be a one-step method or a two-step method, and the labeled antibody is not limited to Fab'-HRP. Depending on the purpose of the measurement, the composition of each reaction buffer or the reaction conditions may be adjusted, such as shortening or lengthening. In addition, human HRF used as a standard can be purified from tissue culture supernatant, cell culture supernatant, or recombinants expressed by the content described in Example 1 or other methods. Purification can be achieved by ion exchange, gel filtration, affinity chromatography using an anti-human HRF antibody, or a combination of various other affinity chromatography....

Embodiment 4

[0189] Western blot

[0190] The culture supernatant of cells or tissues expressing human HRF and the purified recombinant human HRF are separated by 10%-15% SDS-PAGE under reducing conditions and transferred to polyvinylidene difluoride (PVDF) membrane (MILLIPORE). Then use TBS (blocking buffer) containing 5% BSA or 5% skimmed milk powder and 0.05% sodium azide to block at room temperature for 0.5-1 hour, treat with HRF-GKL, and incubate at 25°C for 6 hours or less. Each membrane was washed 4 times with TBS (containing 0.05% sodium azide) containing 0.1% Tween 20, and the bound antibody was mixed with HRP-bound anti-rabbit immunoglobulin antibody diluted 1:1000 with blocking buffer at 25°C. React for 1 hour. After the reaction, the membrane was washed 4 times with TBS containing 0.1% Tween20 (containing 0.05% sodium azide), and the bound antibody was detected by Enhanced chemiluminescence (ECL, Amersham Pharmacia).

[0191] In addition, using HRF-TPY, according to the meth...

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Abstract

The content of a histamine-releasing factor (HRF) protein in a biological sample of a subject is measured and the HRF protein content is compared with that of a normal biological sample. An HRF protein content considerably higher than that of the normal biological sample is employed as an indication of a disease relating to endometriosis or a risk thereof.

Description

technical field [0001] The present invention relates to a diagnostic method of molecular biology for diseases related to endometriosis. Furthermore, the present invention relates to therapeutic drugs and methods for treating endometriosis utilizing the molecular mechanism of this disease. Background technique [0002] Endometriosis is generally a gynecological disease that affects 10% of the female population of reproductive age (Non-Patent Document 1). Endometriotic tissue, like normal endometrium, undergoes periodic proliferation and destruction, resulting in periodic dysmenorrhea, dyspareunia, pelvic pain, and hematuria during menstruation. Furthermore, it is reported that 30 to 40% of infertile patients suffer from this disease (Non-Patent Document 2). The mechanism of endometrial cell transfer and proliferation at other locations in some patients is still unclear, but deregulation of inflammatory cytokines may lead to the development of endometriosis (non-patent liter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53C07K16/18A61K48/00C07K14/52G01N33/68
CPCC12N2799/027G01N33/6893C07K14/52A61K48/00C07K16/18G01N33/6863G01N33/53G01N33/577C12N5/10
Inventor 小杉好纪黑田雅彦及川恒辅大林彻也
Owner PERIODOCK
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