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Cultivation regenerating method for wheatgrass mature embryo tissue

A tissue culture and mature embryo technology is applied in the field of inducing and regenerating plants of ice grass mature embryos. The effect of improving the recovery rate

Inactive Publication Date: 2006-08-23
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the long growth cycle of most forage grasses and the complex inheritance of traits, it is difficult to breed new varieties by conventional breeding methods; resulting in a small number of forage grass production varieties in my country, poor quality, and the level of stress resistance is not high

Method used

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  • Cultivation regenerating method for wheatgrass mature embryo tissue

Examples

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Comparison scheme
Effect test

Embodiment 1

[0009] Embodiment 1: The regeneration method of wheatgrass mature embryo tissue culture includes the following steps,

[0010] (1): Selection and treatment of seeds: select vigorous and plump seeds, soak the seeds in water at room temperature for 2-4 hours, peel off the inner and outer palea, and put them in a solution containing 1.0mg / L 2,4-D Soak for 1.5 hours, then disinfect with 75% alcohol for 30 seconds on the ultra-clean workbench, rinse with sterile water for at least 3 times, then disinfect with 0.2% mercury liter for 5 minutes, and then rinse with sterile water for at least 3 times; In a petri dish with wet sterile filter paper, use a dissecting needle to pick out the embryos with as little endosperm as possible from the scutellum; when the embryos are stripped, the embryos are properly clipped and inoculated on the callus induction medium, 50 grain mature embryo;

[0011] (2): Mature embryos were inoculated on callus induction medium and cultured in the dark at 26°...

Embodiment 2

[0015] Embodiment 2: The regeneration method of mature embryo tissue culture of wheatgrass comprises the following steps,

[0016] (1): Seed selection and treatment, select vigorous and plump seeds, soak the seeds in water at room temperature for 2-4 hours, peel off the inner and outer palea, and put them in a solution containing 3.0mg / L 2,4-D Soak for 3 hours, then disinfect with 75% alcohol for 30 seconds on the ultra-clean workbench, rinse with sterile water for at least 3 times, then disinfect with 30% sodium hypochlorite for about 45 minutes, and then rinse with sterile water for at least 3 times; In the petri dish of wet sterile filter paper, cut off the part containing the embryo with a blade, inoculate it on the callus induction medium, and inoculate 50 mature embryos in each dish;

[0017] (2): Mature embryos were inoculated on callus induction medium and cultured in the dark at 26°C for 14 days, wherein the callus induction medium was: MS basic medium+0.5mol / L mannit...

Embodiment 3

[0021] Embodiment 3: The regeneration method of mature embryo tissue culture of wheatgrass comprises the following steps,

[0022] (1): Seed selection and treatment, select vigorous and plump seeds, soak the seeds in water at room temperature for 2-4 hours, peel off the inner and outer palea, and put them in a solution containing 2.0mg / L 2,4-D Soak for 2 hours, then disinfect with 75% alcohol for 30 seconds on the ultra-clean workbench, rinse with sterile water for at least 3 times, then disinfect with 50% sodium hypochlorite for about 25 minutes, and then rinse with sterile water for at least 3 times; In a petri dish with wet sterile filter paper, use a dissecting needle to pick out the embryos with as little endosperm as possible from the scutellum; when the embryos are stripped, the embryos are properly clipped and inoculated on the callus induction medium, 50 grain mature embryo;

[0023] (2): Mature embryos were inoculated on the callus induction medium and cultured in t...

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Abstract

The present invention relates to mature embryo tissue cultivating regeneration method of wheatgrass. During seed selection and treatment, plump seed with the lemma and the palea eliminated is first soaked in solution containing 2, 4-D in 1.0-4.0 mg / L for 1.5-3.0 hr and sterilized, and the mature embryo is then inoculated to callus inducing culture medicine with increased AgNO3 solution to culture. The present invention has the advantages of capacity of promoting the generation one callus organ and somatic cell to raise callus generating rate, capacity of promoting the generation of seed bud, and capacity of increasing the number of adventitious bud the explant generates and raising the regeneration frequency of the plant.

Description

Technical field: [0001] The invention relates to a tissue culture regeneration method of mature wheatgrass embryos, in particular to a cultivation method for induced plant regeneration of wheatgrass mature embryos. Background technique: [0002] my country's grassland is the largest green space ecosystem in the country, but it is mainly located in the northwest border, most of which belong to arid and semi-arid areas, and the ecological environment is fragile. In recent decades, due to reasons such as overgrazing, irrational use, and deforestation, the phenomenon of "three transformations" of grassland has become increasingly serious. The land resources available for development and utilization in these areas are very rich, and plants with strong drought and salinity resistance can be used to improve the ecological environment in these areas and increase land utilization. Due to the long growth cycle of most forage grasses and the complex inheritance of traits, it is diffic...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01C1/00C12N5/04
Inventor 米福贵云锦凤霍秀文露晓平徐春波魏建华王宏枝李瑞芬张辉刘娟王桂花
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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