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Nano colloid gold reagent strip for immunochromatography detection of bladder carcinoma and detection method thereof

A technology for immunochromatographic detection and bladder cancer, which is applied in biological testing, measuring devices, and analytical materials, can solve the problems of less than 50% positive rate, many influencing factors, and high cost, and achieve high specificity and accuracy, and easy operation Simple, fast, fast detection effect

Inactive Publication Date: 2006-07-12
ZHONGSHAN HOSPITAL XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, urine cytology examination has many influencing factors, and the positive rate is low. For patients with stage I and II, the positive rate is less than 50%.
However, the cost is too high, it cannot be used for bedside examination, and expensive electrochemiluminescence instrument is required, which is not suitable for the medical conditions of primary hospitals and backward areas

Method used

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  • Nano colloid gold reagent strip for immunochromatography detection of bladder carcinoma and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] See figure 1 The bladder cancer immunochromatographic detection reagent strip is equipped with a hard paper bottom plate 1. On the bottom plate 1, there are a urine-absorbing glass fiber layer 2, a polyester film layer 3, and a gold-labeled anti-CK19 antibody (Monoclonal Antibody toCytokeratin 19, clone: ​​A53-B / A2) glass fiber layer 4, nitrocellulose membrane layer 5 (NC membrane) and absorbent filter paper layer 6. The layers are sequentially and alternately bonded to the cardboard base 1 and assembled. On the nitrocellulose membrane layer 5, there are a T test area 7 and a C quality control area 8. Test area 7 is a zone labeled Ks19.2 antibody (Clone Determination Ks19.2 (Z105.6), also published as BM 19.21, MAK 19.21), and quality control zone 8 is a zone labeled anti-mouse secondary antibody. The urine-absorbent glass fiber layer 2 (zone) is used as the sample application zone. The size of the nitrocellulose film layer 5 is: length 2.5 cm, width 3 mm, and the bottom pl...

Embodiment 2

[0038] See figure 1 Similar to Example 1, the difference is that the Ks19.2 antibody (Clone Determination Ks 19.2 (Z105.6), also published as BM 19.21, MAK 19.21) is fixed on the glass fiber layer 4, and the nitrocellulose film layer 5 is The test area is marked with a gold-labeled anti-CK19 antibody (Monoclonal Antibody to Cytokeratin 19, clone: ​​A53-B / A2) zone. The size of the nitrocellulose film layer 5 is 2 cm in length and 3.5 mm in width.

[0039] When performing immunochromatographic detection of bladder cancer, first take 100 μL of the sample to be tested and add it to the sample application area of ​​the reagent strip, namely the urine-absorbent glass fiber layer 2, and observe and record the test results within 2 minutes. The judgment of the detection result is the same as in Example 1.

Embodiment 3

[0041] See figure 1 , Similar to Example 2, the difference is that the size of the nitrocellulose film layer 5 is: length 4.5 cm, width 4.5 mm.

[0042] When performing immunochromatographic detection of bladder cancer, first take 30 μL of the sample to be tested and add it to the sample loading area of ​​the reagent strip, namely the urine-absorbent glass fiber layer 2, and observe and record the test results within 15 minutes. The judgment of the detection result is the same as in Example 1.

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Abstract

The nano CGIA test strip and testing method for bladder carcinoma combines ICG method and nano CG technique. Wherein, it is prepared by: overlaying and sticking on the all of an absorption-urine glass fiber layer, a polyester film layer, a glass fiber layer fixed gold-labeled baseboard anti-CK19 antibody or anti-Ks19.2 antibody, a CN film layer with a T testing zone enveloped said antibody and a C mass control zone enveloped sheep antibody and rat antibody, and an absorption-water filter paper layer.

Description

Technical field [0001] The invention relates to a urine detection appliance, in particular to a reagent strip for detecting bladder cancer using immuno-chromatography and nano colloidal gold technology and a detection method thereof. Background technique [0002] Immunochromatography is a rapid diagnostic technique that has emerged in recent years. Its principle is to fix specific antibodies on a certain zone of the nitrocellulose membrane. When one end of the dry nitrocellulose membrane is immersed in the sample, due to the capillary Action, the sample will move forward along the film. When moving to the area where the antibody is immobilized, the corresponding antigen in the sample specifically binds to the antibody. If using immunogold or immunoenzyme staining, the area can show a certain color, so as to realize specific immunodiagnosis. Its advantages are simplified operation steps, faster detection speed, good specificity and high sensitivity, and it is suitable for general ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/53G01N33/52
Inventor 张忠英葛金梅游攀叶辉铭
Owner ZHONGSHAN HOSPITAL XIAMEN UNIV
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