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Method for preparing bacillus thuringiensis microbiological pesticide by starch waste liquor

A microbial insecticide, the technology of Bacillus thuringiensis, is applied in the field of production of Bacillus thuringiensis microbial insecticides, which can solve the problems of not yet seen Bacillus thuringiensis microbial insecticides, limited application scope, and high nucleic acid content, and achieves expansion of resource utilization. Ways, promotion and application, high value-added effect

Inactive Publication Date: 2006-07-12
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this kind of SCP feed also has some defects: such as high nucleic acid content (eating too much may cause diseases such as gout), no meat quality, and poor flavor limit its application range
[0007] So far, no reports have been found on the use of starch wastewater as raw material to ferment and produce Bacillus thuringiensis microbial insecticides through domestic and foreign literature and patent searches

Method used

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  • Method for preparing bacillus thuringiensis microbiological pesticide by starch waste liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 produces water suspension concentrate:

[0029] (1) Starch wastewater sample

[0030]The starch wastewater from a starch factory in Shijiazhuang City, Hebei Province is taken. The factory uses sweet potatoes as raw materials to produce starch. The processing process is: sweet potatoes→washing→grinding→sieving→slurry→centrifugal separation→starch washing→dehydration→drying→starch. The main sources of wastewater are washing water and separation wastewater (potato pulp wastewater and protein water). After analysis, the carbohydrate content in the waste water was 13.2g / L, the crude protein content was 6.1g / L, and the pH=4.45.

[0031] (2) Medium conditioning

[0032] Adjust the solid content of starch wastewater to 3.5%, add 0.5g / L of dipotassium hydrogen phosphate mineral salt, 0.2g / L of magnesium sulfate, 0.02g / L of manganese sulfate and 2.0g / L of defoaming agent foam enemy. Use NaOH to adjust the pH to 9.7-9.8, sterilize in the seed tank and fermenter at ...

Embodiment 2

[0044] Example 2 Production of wettable powder

[0045] (1) Starch wastewater sample

[0046] With embodiment 1.

[0047] (2) Medium conditioning

[0048] With embodiment 1.

[0049] (3) Shake flask culture

[0050] With embodiment 1.

[0051] (4) Seed tank fermentation

[0052] Inoculate 2% of the above-mentioned shake flask bacterial liquid, and ferment and cultivate. The culture conditions are: tank temperature 28° C., tank pressure 0.03 MPa, ventilation rate 1:1.0 (V / V / min), stirring speed 240 rpm, and fermentation time 12 hours.

[0053] (5) Fermentation tank fermentation

[0054] Inoculate the fermenter with 8% seed solution. The culture conditions are: tank temperature 28° C., tank pressure 0.03 MPa, ventilation rate 1:1.2 (V / V / min), stirring speed 200 rpm, and fermentation time 36 hours.

[0055] (6) post-treatment of fermentation broth

[0056] After the fermentation is finished, concentrate 5 times by high-speed centrifugation, add 40g / L dispersant kaolin a...

Embodiment 3

[0059] Example 3 Production of wettable powder

[0060] (1) Starch wastewater sample

[0061] Take starch wastewater from a starch factory in Beijing. The factory uses jade as raw material for raw starch. The processing process is: corn→sieving→soaking→crushing→germ separation→grinding→sieving→precipitation separation→starch washing→dehydration→ Dry → starch. The main sources of wastewater are soaking water and yellow slurry water. After analysis, the carbohydrate content in the starch wastewater was 6.8g / L, the crude protein content was 19.0g / L, and the pH=4.80.

[0062] (2) Medium conditioning

[0063] Adjust the solid content of starch wastewater to 5.0%, add 0.75g / L dipotassium hydrogen phosphate, 0.3g / L magnesium sulfate, 0.03g / L manganese sulfate and 2.5g / L foam enemy. Adjust the pH to 9.5 with KOH, sterilize in the seed tank and fermenter at 15 lb pressure and 121°C for 60 minutes, cool to 35°C for later use, and the pH after sterilization is about 7.0-8.0.

[0064...

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Abstract

The invention provides a process for preparing Bacillus thuringiensis microbial insecticide from starch waste-water through liquid submerged cultuse, which comprises the steps of culture medium conditioning, bottle-shaking cultivation, seed tank fermentation, fermentation tank fermenting and fermentation liquid post-treatment.

Description

technical field [0001] The invention relates to resource utilization of starch wastewater in environmental engineering, in particular to a method for producing Bacillus thuringiensis (abbreviated as Bacillus thuringiensis) microbial insecticide by using the wastewater generated in the starch processing process. Background technique [0002] Starch processing wastewater (Starch processing wastewater) is a kind of high-concentration organic wastewater generated during starch processing. These wastewater contain a large amount of residual starch, glucose, fructose, maltose and other carbohydrates, proteins and organic acids, etc., which are easy to rot and smell , COD is usually between 2000-20000mg / L, and it is one of the most polluted wastewater in the food industry. Most of the organic matter contained in starch wastewater is a valuable resource that can be recycled. For example, corn starch wastewater generally contains 0.3-0.7% total sugar, about 2.1% crude protein, 2-3% ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02
Inventor 周顺桂倪晋仁张锦涛常明卢娜
Owner PEKING UNIV
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