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Fusion protein of tetanus toxin T cell expressing bit polypeptide and human bata lymph cell stimulating factor and preparing thereof

A technology of tetanus toxin and B lymphocytes, applied in the direction of peptides, hybrid peptides, specific peptides, etc., can solve problems such as the lack of effective methods for diseases, achieve good protection, prevent EAE, and reduce toxic side effects.

Inactive Publication Date: 2006-06-28
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0016] At present, there is no effective method for the treatment of this disease, mainly injection of alloantibodies

Method used

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  • Fusion protein of tetanus toxin T cell expressing bit polypeptide and human bata lymph cell stimulating factor and preparing thereof
  • Fusion protein of tetanus toxin T cell expressing bit polypeptide and human bata lymph cell stimulating factor and preparing thereof
  • Fusion protein of tetanus toxin T cell expressing bit polypeptide and human bata lymph cell stimulating factor and preparing thereof

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Embodiment Construction

[0042] According to the technical scheme of the present invention, the T cell epitope polypeptide of tetanus toxin and human B lymphocyte stimulating factor fusion protein, select the T cell epitope polypeptide of tetanus toxin-Gln-Tyr-Ile-Lys-Ala-Asn-Ser -Lys-Phe-Ile-Gly-Ile-Thr-Glu-(Glutamine-Tyrosine-Isoleucine-Lysine-Alanine-Asparagine-Serine-Lysine-Phenylalanine acid-isoleucine-glycine-isoleucine-threonine-glutamic acid), which can stimulate the body's CD4 + cellular response. The TT-BLyS fusion gene was constructed by step-by-step gene cloning, and was highly expressed in Escherichia coli. The expression product is cleaved by lysozyme, washed by inclusion body and purified by gel filtration chromatography column, and the protein purity can reach more than 95%. After subcutaneous injection of TT-BLyS, high-level antibody responses were induced in New Zealand rabbits, rats, and economical mice. The polyclonal antibody can neutralize the stimulating effect of natural BLyS...

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Abstract

The invention discloses tetanus toxin T cell epi position peptide and human B lymphocyte activate and gene interfusing albumen. It could activate CD4+T cell to take cell action, promote body liquid immunity level. It adopts step clone to construct TT and BLyS interfusing gene and gain high efficiency expression in coliform bacteria. It conquers the immunity tolerate of the body.

Description

technical field [0001] The invention belongs to the technology of gene cloning, gene recombination, expression of exogenous gene in prokaryotic cells, purification of target protein, in vitro activity measurement of recombinant protein vaccine and identification of physical and chemical properties in the field of medical biotechnology, and relates to an anti-autoimmune Disease medicine and preparation method thereof, in particular to B lymphocyte stimulating factor recombinant protein vaccine composed of tetanus toxin T cell epitope polypeptide and human B lymphocyte stimulating factor fusion protein (TT-BLyS) and preparation method thereof. Background technique [0002] 1.1 Related research on B lymphocyte stimulating factors [0003] B lymphocyte stimulating factor is a new member of the tumor necrosis factor (TumorNecrosis Factor, TNF) family discovered by Swiss and American scientists in 1999. This molecule was discovered by many different ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/63
Inventor 张英起薛晓畅颜真赵宁
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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