Quick detecting reagent for gonococcus

A technology for detecting reagents and Neisseria gonorrhoeae, applied in the field of Neisseria gonorrhoeae, can solve problems such as false positives, restricted development, and false negatives

Inactive Publication Date: 2006-06-07
广州蓝星生物科技开发有限公司
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  • Claims
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AI Technical Summary

Problems solved by technology

This method has high sensitivity and strong specificity, but it has high requirements on the quality of the required reagents and technical personnel, and has a very high false positive and false negative rate, which limits its development in primary hospitals.

Method used

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Embodiment Construction

[0017] The present invention is used to detect the gonorrhoeae that exists in the patient's urethral secretions, and is determined by the joint detection of oxidase test and proline aminopeptidase test, and specific implementation steps are as follows:

[0018] 1. Oxidase test steps:

[0019] aUse a clean sterile cotton swab to collect secretions from the patient's urethral opening.

[0020] b Squeeze the cotton swab stained with secretions gently on the glass slide to spread the secretion on the slide.

[0021] c. Add a drop of oxidase reaction reagent on the secretion. If the secretion changes from colorless to purple within five minutes, it is positive, and if it does not change color, it is negative.

[0022] 2. Proline aminopeptidase test procedure.

[0023] a Add 560ul of reagent A to the reaction bottle.

[0024] b Use a clean sterile cotton swab to collect secretions from the patient's urethral opening.

[0025] c. Put the cotton swab with secretions into the react...

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Abstract

The quick gonococcus detecting reagent consists of separately stored oxidase reaction reagent and proline aminopeptidase reaction reagent. The oxidase reaction reagent is water solution of tetramthyl-p-phenylene diamine of 10 g / l concentration, and the proline aminopeptidase reaction reagent consists of separately stored six kinds of reagents, including reagent A of 2 g / l concentration L-proline-P-nitroaniline aqua, reagent B of 0.01 mol / l PBS, reagent C of 2.5 mol / l hydrochloric acid aqua, reagent D of 11 g / l concentration sodium nitrite aqua, reagent E of 5 g / l ammonium amino sulfonate aqua, and reagent F of 0.55 g / l concentration ethanol solution of N-(1-naphthyl)ethylenediamino dichloride. The present invention is used in detecting gonococcus in urethra excreta according to positive of both gonococcus oxidase test and proline aminopeptidase test.

Description

Technical field: [0001] The invention is used for detecting gonorrhea in urethral secretions of patients, and belongs to the field of biochemical diagnostic reagents. Background technique: [0002] Gonorrhea is one of the most common sexually transmitted diseases. It is widely prevalent in the world, causing worldwide danger and causing great harm to human health. The pathogen of gonorrhea is Neisseria gonorrhoeae, also known as gonorrhoeae. Humans are the only natural host of gonorrhea, which mainly infects mucous membranes, especially the urethral mucous membranes. Neisseria gonorrhoeae invade the male and female urethras, and rely on adhesion factors to adhere to the surface of epithelial cells to reproduce. After gonococcal infection, about 1% of males have no symptoms, and 75% of female patients are asymptomatic. Therefore, the misdiagnosis rate is extremely high, especially for females, based on the presence or absence of clinical symptoms alone. Accurate and reliabl...

Claims

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Application Information

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IPC IPC(8): C12Q1/26C12Q1/04C12Q1/25
Inventor 王虹
Owner 广州蓝星生物科技开发有限公司
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