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Bioinformatic screening process of simulated epitope of pathogenic microbe

A technology of pathogenic microorganisms and bioinformatics, applied in the field of protective antigen mimic epitopes, can solve the problems of slow progress in the research of pathogenic microorganism antigen protein analysis, tedious manpower and material resources, and huge cost

Inactive Publication Date: 2006-05-17
THE FIRST INST OF OCEANOGRAPHY SOA
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Problems solved by technology

The above-mentioned methods all have the disadvantages of time-consuming, lengthy, and huge manpower and material resources; at the same time, a large number of special reagents and instruments are required in the identification process, which is difficult for most laboratories to accept. reason

Method used

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  • Bioinformatic screening process of simulated epitope of pathogenic microbe
  • Bioinformatic screening process of simulated epitope of pathogenic microbe
  • Bioinformatic screening process of simulated epitope of pathogenic microbe

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Embodiment Construction

[0019] In this method, different isolates of lymphocyst virus (LCDV-1, LCDV-cn) are used as model organisms, firstly, the genome sequence of LCDV-1 is compared by BLAST, and two protective antigen protein candidate genes TK and ORF29 are screened; Then, the hydrophobicity and hydrophilicity, antigenicity and transmembrane structure analysis of the protein structure and properties expressed by the two genes were carried out, and the results of the above structure and property analysis were combined, and finally the unknown LCDV-cn was deduced by the calculation of the comprehensive antigenic index. Mimic epitopes.

[0020] The mimotopes obtained above were artificially synthesized, and the immunoreactivity of various mimotopes was tested by competitive enzyme-linked immunosorbent assay.

[0021] The specific method is as follows:

[0022] 1. BLAST search and analysis of antigenic protein

[0023] By performing BLAST analysis (http: / / www.ncbi.nlm.nih.gov / blast, the parameters ...

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Abstract

The bioinformatic screening process of simulated epitope of pathogenic microbe includes the first BLAST comparison of LCDV-1 genome sequence to screen out protective antigen protein candidate gene TK and ORF29; the subsequent hydrophobicity, hydrophilicity, antigenicity and transmembrane structure analysis of these two gene expressed proteins and synthesizing 12 kinds of structural analysis results; and final presumption of unknown LCDV-cn corresponding simulated epitopes with I>0 sequences based on the comprehensive antigen index formula. The obtained simulated epitopes are further artificially synthesized and the immunoreactivity of various simulated epitopes are inspected through competitive enzyme-linked immune analysis. The present invention makes best utilization of rich nucleic acid and protein sequence resource to capture the simulated epitope of unknown pathogenic microbe antigen protein through specific bioinformatic analysis.

Description

technical field [0001] The present invention relates to the bioinformatics analysis of the known gene / genome sequence to quickly obtain the protective antigen mimic epitopes of pathogenic microorganisms with unknown genome or antigenic information, that is, the bioinformatics screening method of pathogenic microorganism mimic epitopes. Background technique [0002] The emergence of vaccines has made a great contribution to the health of humans and farmed organisms. With the development of science and technology, the technical level of vaccine development is also constantly improving. The earliest inactivated vaccine invented is still widely used today. Its biggest defect is that the stimulated immune response is not perfect, and it is difficult to provide effective immune protection. However, the attenuated vaccine has serious safety problems. restricts its application. In this situation, the third-generation subunit vaccine and the fourth-generation nucleic acid vaccine d...

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 吴谡琦孙修勤张进兴郑凤荣洪旭光曲凌云
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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