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Separated soybean protein preparation process and prepared soybean small peptide and process

A technology for soybean protein isolate and preparation process, which is applied to the preparation methods of peptides, peptides, peptide sources, etc., can solve the problems of affecting the extraction yield, low single-enzyme hydrolysis rate, cumbersome production process, etc., so as to improve the enzymatic hydrolysis efficiency. , The effect of shortening the enzymatic hydrolysis time and reducing the cost

Inactive Publication Date: 2006-05-03
黄磊 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since sodium hydroxide is a strong base, using it to adjust the pH value has the following disadvantages: ①The soybean meal powder particles swell too much, which partially causes peptization and affects the extraction yield. Generally, the yield is below 45% (relative to the soybean meal raw material) ; ② affect the filtration speed during separation; ③ increase the washing difficulty when bean dregs are used as dietary fiber; ④ affect the hydrolysis degree and yield of soybean small peptide enzymolysis
In addition, in the prior art of producing soybean small peptides by enzymatic hydrolysis, single-enzyme or multi-enzyme mixed enzymolysis is mostly used, and the hydrolysis rate of single-enzyme hydrolysis is low, while the multi-enzyme hydrolysis currently used all uses self-prepared mixed enzymes or multiple enzymes. The enzymes are added separately, which not only increases the production cost, but also makes the production process cumbersome

Method used

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Examples

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Effect test

Embodiment 1

[0027] Edible soybean meal 100Kg, crushed to 30 mesh, added 600 kg of water, stirred evenly, adjusted pH 8.0 with 15% ammonium hydroxide solution, extracted at 40°C for 2 hours, separated and precipitated to obtain primary extract and slag. Add 600 kg of water to the slag, adjust the pH to 8.0 with 15% ammonium hydroxide solution, extract at 40°C for 2 hours, separate the extract, and combine the secondary extracts. The extract was adjusted to PH4.0 with 15% hydrochloric acid, stirred evenly and left to stand, separated and precipitated to obtain wet soybean protein isolate, which was washed with deionized water, and prepared into a turbid solution with a solid content of 15%. Homogenize and spray-dry to obtain 49.8Kg of soybean protein isolate, the protein content is ≥ 90%, and the yield is 49.8%.

Embodiment 2

[0029] Edible soybean meal 100Kg, crushed to 50 mesh, added 800 kg of water, stirred evenly, adjusted pH9 with 18% ammonium hydroxide solution, extracted at 50°C for 1 hour, separated and precipitated to obtain primary extract and slag. Add 800 kg of water to the slag, adjust the pH to 9 with 18% ammonium hydroxide solution, extract at 50°C for 1 hour, separate the extract, and combine the secondary extracts. Use 18% citric acid in the extract, adjust the pH to 4.5, stir well and let it stand, separate the precipitate to obtain wet soybean protein isolate, which is dispersed and washed with deionized water, and prepared into a turbid solid content of 23%. liquid, homogeneous, and spray-dried to obtain 49.5Kg of soybean protein isolate, with a protein content of ≥90% and a yield of 49.5%.

Embodiment 3

[0031] Edible soybean meal 100Kg, crushed to 60 mesh, added 1000 kg of water, stirred evenly, adjusted pH 10.5 with 20% ammonium hydroxide solution, extracted at 65°C for 2 hours, separated and precipitated to obtain primary extract and slag. Use 20% ammonium hydroxide solution to adjust the pH to 10.5, extract at 65°C for 2 hours, separate the extract, and combine the secondary extracts; use 20% hydrochloric acid or citric acid to adjust the pH to 5.0, precipitate, and separate to get wet Soybean protein isolate, the wet soy protein isolate is dispersed and washed with deionized water, prepared into a turbid liquid with a solid content of 30%, homogeneous, spray-dried to obtain 50.1Kg of soybean protein isolate, protein content ≥ 90%, and a yield of 50.1%. .

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Abstract

The invention discloses a new synthesis method for bicetyl pentaerythritol diphosphite ester, which comprises two steps shown below: 1. synthesis to pentaerythritol diphosphite ester; 2. synethsis to the product. This invention needs no organic solvent and generates no phenol, so environmental friendly.

Description

technical field [0001] The invention relates to the field of soybean deep processing, in particular to a method for producing soybean protein isolate and soybean small peptide and a soybean small peptide product. Background technique [0002] Soybean protein isolate is rich in eight essential amino acids such as lysine, tryptophan and valine, and other non-essential amino acids. The amino acid composition ratio meets the requirements of the World Health Organization (WHO). . Soybean protein isolate has the characteristics of water retention, adhesion, gelation, oil absorption and emulsification. It is widely used as the basic raw material of the food industry, and can also directly enter the consumer market to provide consumers with high-quality plant protein. It can also be used in feed processing , pharmaceutical, plastic industry and other fields are widely used. Soybean protein small peptide is a soybean protein fragment with a molecular weight below 1000D consisting o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K4/10C07K14/415C12P21/00
Inventor 黄磊林坚
Owner 黄磊
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