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Microorganism additive of alfalfa ensilage

A technology of microbial additives and additives, applied in the direction of microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of affecting fermentation and reducing the pH value of silage, and achieve the effect of improving the quality of silage and reducing the pH value

Inactive Publication Date: 2006-04-12
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in addition to a variety of lactic acid bacteria, the green juice fermentation broth also contains a variety of harmful bacterial species. These bacteria also have the role of initiating factors, inducing the expansion of harmful bacterial populations in the process of alfalfa silage, thereby reducing or slowing down The green juice fermented liquid can better promote the expansion and reproduction of lactic acid bacteria, so that the pH value of the silage can not be sharply reduced in a short period of time during the pre-fermentation period and the early acidification stage of the silage alfalfa. This is the green juice fermented liquid used as a silage additive in alfalfa silage The biggest defect that affects the high-quality fermentation of silage alfalfa and the formation of high-quality silage
In further searches, there has been no report about isolating Streptococcus lactis (Streptococcus lactis) from alfalfa as a silage additive so far, nor has there been a report on mixing green juice fermentation broth and Streptococcus lactis as an alfalfa silage additive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment one: in the alfalfa silage microbial additive, the green juice fermented liquid: the living streptococcus lactis in the MRS liquid medium is 50%: 50%, and this additive of every 10ml contains PFJ 5ml, streptococcus lactis 10 8 cuf, add 10ml additive per kilogram of alfalfa when silage.

[0011] 1. Select fresh alfalfa materials: take fresh alfalfa plants, remove about 10cm from the base and the top, remove the main branches and thicker branches at the same time, and keep the remaining young branches and leaves as the production material of green juice fermentation liquid;

[0012] 2. Production of green juice fermentation liquid: chop fresh alfalfa material into 3-5cm, add distilled water according to the ratio of alfalfa (g) and water (ml) 1:2.5, mix thoroughly, mash with a high-speed tissue grinder, statically Place for 1 hour, filter with double-layer gauze, add glucose or syrup with an equivalent amount of glucose in the filtrate according to the ratio of...

Embodiment 2

[0021] Embodiment two: in the alfalfa silage microbial additive, the green juice fermented liquid: the living streptococcus lactis in the MRS liquid medium is the ratio of 60%: 40% mixes, forms the alfalfa silage microbial additive, wherein every 10ml this additive contains PFJ 6ml, Lactococcus 8×10 7 cuf, add 12ml additive per kilogram of alfalfa when silage.

[0022] 1. Select fresh alfalfa materials: take fresh alfalfa plants, remove about 10cm from the base and the top, remove the main branches and thicker branches at the same time, and keep the remaining young branches and leaves as the production material of green juice fermentation liquid;

[0023] 2. Production of green juice fermentation liquid: chop fresh alfalfa material into 3-5cm, add distilled water according to the ratio of alfalfa (g) and water (ml) 1:2.5, mix thoroughly, mash with a high-speed tissue grinder, statically Place for 1 hour, filter with double-layer gauze, add glucose or syrup with an equivalent ...

Embodiment 3

[0032] Embodiment three: in the alfalfa silage microbial additive, the green juice fermented liquid: the living streptococcus lactis in the MRS liquid medium is 70%: 30% ratio is mixed, forms the alfalfa silage microbial additive, wherein every 10ml this additive contains PFJ 7ml, Lactococcus 6×10 7 cuf, add 15ml additive per kilogram of alfalfa when silage.

[0033] 1. Select fresh alfalfa materials: take fresh alfalfa plants, remove about 10cm from the base and the top, remove the main branches and thicker branches at the same time, and keep the remaining young branches and leaves as the production material of green juice fermentation liquid;

[0034] 2. Production of green juice fermentation liquid: chop fresh alfalfa material into 3-5cm, add distilled water according to the ratio of alfalfa (g) and water (ml) 1:2.5, mix thoroughly, mash with a high-speed tissue grinder, statically Place for 1 hour, filter with double-layer gauze, add glucose or syrup with an equivalent am...

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PUM

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Abstract

A microbial additive for preparing the ensilage of alfalfa is prepared from the fermented liquid of green juice and the liquid culture medium MRS containing lactostreptococcus in the ratio of (50-70): (30-50). It can obviously lower the pH value of ensilage and increase its quality.

Description

technical field [0001] The invention relates to an additive in the field of biotechnology, in particular to a microbial additive for alfalfa silage. Background technique [0002] Alfalfa (Medicago sativa L. hereinafter referred to as alfalfa) is a perennial leguminous forage with rich nutritional value. It is a high-quality forage for herbivores and has the reputation of "the king of forage". For a long time, alfalfa has been preserved mainly by sun-dried hay. The biggest disadvantage of this method is the serious loss of nutrients. In recent years, Canada, Japan and other countries have begun to use the method of silage to preserve alfalfa, which has solved the problem of serious loss of nutrients in the process of alfalfa preservation. Because alfalfa has low soluble sugar content and high buffer energy, it is a legume forage that is difficult to silage, and the direct silage effect is not good, so it is necessary to use additives in the silage fermentation process. The ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K3/03C12R1/46A23K30/18
Inventor 安渊田瑞霞王光文
Owner SHANGHAI JIAO TONG UNIV
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