Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Modular antigen transporter molecules (MAT molecules) for modulating immune reactions, associated constructs, methods and uses

An immunoglobulin, modular technology, applied in chemical instruments and methods, biochemical equipment and methods, antibody medical components, etc., can solve problems such as allergic reactions and desensitization

Active Publication Date: 2006-02-22
BOEHRINGER LNGELHEIM VETMEDICA GMBH
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Although it is possible and intentional to use antigens as cargo molecules, it has so far not been possible to generate in an individual an immune response sufficient to achieve specific requirements in terms of targeting and dosage, e. Require

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Modular antigen transporter molecules (MAT molecules) for modulating immune reactions, associated constructs, methods and uses
  • Modular antigen transporter molecules (MAT molecules) for modulating immune reactions, associated constructs, methods and uses
  • Modular antigen transporter molecules (MAT molecules) for modulating immune reactions, associated constructs, methods and uses

Examples

Experimental program
Comparison scheme
Effect test

example 28

[0093] spacer module

[0094] Spacer modules which can be used in the present invention are all molecules which are suitable for coupling other modules which are components of the MAT molecule to each other. Coupling can be carried out both covalently and non-covalently. The role of the spacer module is to spatially separate the various modules of the MAT molecule from each other such that they do not adversely affect each other's respective functions. The modules of the MAT molecule of the invention can be coupled via spacer modules which can then be cleaved again by chemical or enzymatic reactions, for example by proteases. In this way, the modules of MAT molecules connected by spacer modules can be separated from each other again when required.

[0095] In general, all currently known or future known proteases can be used in the present invention [29, 30]. Proteases commonly used today are thrombin, factor Xa, enterokinase or the TAGZyme system (Qiagen, Hilden, Germany),...

Embodiment 1

[0158] Example 1: Cloning of expression vectors for MAT molecules

[0159] All molecular biology methods described below were performed according to standard methods known to those skilled in the art [43]. Vector pQE-30 (Qiagen, Hilden, Germany) was used to clone the vector for expression of MAT molecule (modular antigen transport molecule).

[0160]In the first step, the nucleic acid sequence encoding the transport module is introduced into a bacterial expression vector. The DNA sequence encoding the amino acid GYGRKKRRQRRR of HIV Tat was introduced into vector pQE-30 in the form of a synthetic oligonucleotide. In addition to the HIV Tat sequence, the oligonucleotide contains a recognition sequence for the restriction enzyme Bgl II at the 5' end and recognition sequences for BamH I, Spe I, Pst I and Hind III at the 3' end. Subsequently, the synthesized HIV Tat sequence was digested with Bgl II and Hind III, and the vector pQE-30 was digested with restriction endonucleases B...

Embodiment 2

[0161] The coding sequences for the various antigens in the antigen moiety were isolated by various methods known to those skilled in the art [43]. The Bet v 1 sequence was obtained by synthetic oligonucleotides, the Asp f 1 and Asp f 6 sequences had been obtained in previous studies and were isolated from the vectors used in these studies [45,46] and subsequently introduced into pQE -30 vector, the Der p 1 sequence was isolated by reverse transcriptase PCR using mRNA from the house dust mite (Dermatophagoides pteronyssinus).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

By adding the novel developed modular antigen transporter molecules (MAT molecules) and other instruments related thereto, the immune system of an individual can be effectively modulated in a targeted manner. The MAT molecules contain a translocation module which transports the MAT molecule inside the cell, an active intracellular targeting molecule resulting in intracellular transport of the MAT molecule to the organelles of the cell which are responsible for antigen processing and an antigen module which determines the specificity of the immune response modulated by the MAT molecule. Optionally, other modules such as tag modules or spacer modules can be contained in the MAT molecule. The invention comprises the inventive MAT molecules, antibodies which are produced using said MAT molecules and therapeutic agents and diagnostic reagents which are produced with MAT molecules. The invention also comprises applications containing said MAT molecules, antibodies, therapeutic agents and diagnostic reagents.

Description

technical field [0001] The present invention relates to stimulating and suppressing the immune system to prevent, treat or diagnose diseases associated with under- or over-activation of the immune system, including infectious diseases, neoplastic diseases, allergies, autoimmune diseases, graft rejection React and so on. At the heart of the invention is a novel method of influencing the immune system through a MAT molecule consisting of at least three components: [0002] 1. A transport module (module), its effect is to make the MAT molecule penetrate into the cell from the outside of the cell, [0003] 2. An intracellular targeting module that acts to cause the MAT molecule to be processed intracellularly in order to produce an altered immune response or altered antigen presentation, and [0004] 3. An antigenic moiety that determines the specificity of the modulated immune response. [0005] The combination of these three elements produces a MAT molecule, whereby the immun...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/62A61K39/00C07K14/38
CPCA61K39/00C07K14/38C07K2319/10C07K2319/40C12N15/62C12N2310/111C12N2810/40A61P29/00A61P31/00A61P35/00A61P37/00A61P37/04A61P37/06A61P37/08A61P39/00
Inventor 诺贝特·兰平克拉默瑞·雷托扎比内·弗吕克格尔伊莎贝尔·戴格尔
Owner BOEHRINGER LNGELHEIM VETMEDICA GMBH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com