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Micro flow control chip capillary electrophoresis negative pressure sampling method

A technology of microfluidic chip and capillary electrophoresis, applied in separation methods, chemical instruments and methods, material analysis through electromagnetic means, etc., can solve the problems of infusion equipment with high voltage and affect safe operation, and avoid discrimination effect , improved safety, and improved sample injection speed

Inactive Publication Date: 2006-02-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the conductive sample or electrophoresis buffer leaks out of the infusion device, it will cause the infusion device to be charged with high voltage and affect the safe operation

Method used

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  • Micro flow control chip capillary electrophoresis negative pressure sampling method
  • Micro flow control chip capillary electrophoresis negative pressure sampling method
  • Micro flow control chip capillary electrophoresis negative pressure sampling method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The steps of the microfluidic chip negative pressure sampling capillary electrophoresis analysis method of the present invention are as follows:

[0019] The diagram of the device for capillary electrophoresis separation of negative pressure injection microfluidic chip is shown in figure 1 . Among them, 1 represents the microfluidic chip, the channel between B and BW on the microfluidic chip is the separation channel, the channel between S and SW is the sampling channel, and B, BW, S and SW represent buffer reservoirs respectively , buffer waste liquid reservoir, sample liquid reservoir and sample waste liquid reservoir. 2 represents the syringe pump, 3 is the three-way valve, 4 is the interface, and 5 is the high-voltage power supply. Add the sample solution to the sample reservoir S on the microfluidic chip, add different volumes of electrophoresis buffer to the other reservoirs B, SW, and BW, and keep the liquid levels of the reservoirs B and BW at both ends of the...

Embodiment 2

[0022] Provide a more specific optimization instance according to embodiment 1, see figure 1 , the width of the channel on the microfluidic chip is 10-100 μm, and the depth is 5-50 μm. The channel between S and SW is the sampling channel with a length of 5-50 mm. The channel between B and BW is the separation channel with a length of 10-100mm. The separation channel and the injection channel cross each other, or have a double T structure. Drill small holes at both ends of the sampling channel and the separation channel, and use adhesive to bond the micro plastic liquid storage tank on the small holes. The outer diameter of the plastic liquid storage tank is 6mm, the inner diameter is 4mm, and the height is 6mm. S, SW, B, and BW represent the sample liquid storage pool, the sample waste liquid storage pool, the buffer liquid storage pool, and the buffer liquid waste liquid storage pool, respectively. Add 150 μL of 20 mmol / L borax buffer solution with pH 9.2 to reservoir B and...

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Abstract

This invention relates to one electrophoresis sample in and isolation method of micro flow control capillary, which is characterized by the following: using micro flow control chip network structure; adopting column plug pump and taking sample from waste pool air through three-way valve and interface to form negative pressure; the micro flow chip and other sample solution and buffer liquid flowing downward and forming stable sample plug in the crossing place of sample in channel and isolation channel and connecting the valve with air through three-way valve.

Description

Technical field [0001] The invention relates to a microfluidic chip capillary electrophoresis analysis technology, in particular to a negative pressure sampling and separation technology on a microfluidic chip capillary electrophoresis. Background technique [0002] Since the concept of micro-total analysis system was proposed in 1990, microfluidic chip technology has opened up a broad space for development in the fields of medicine and life sciences. Microfluidic chip capillary electrophoresis technology has been used in DNA sequencing, separation and identification of DNA fragments, separation and determination of amino acids, peptides, proteins, and analysis of components in single cells. [0003] For the above applications, accurate control of the picoliter sample injection volume is the key to separation and determination. At present, electric sampling methods based on electroosmotic flow drive are widely used, such as electro-clamping sampling, suspension sampling and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447B81B1/00
Inventor 殷学锋张磊
Owner ZHEJIANG UNIV
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