Chinese herbal medicinal diluent for cow frozen semen

Abstract

The invention discloses a Chinese herbal medicinal diluent for cow frozen semen, which comprises basic solution and extract of Chinese medicinal herbs, the content of the Chinese medicinal herb extract is 10-14% by volume ratio of the basic solution, 1L of the basic solution contains glucose 15g, lactin 30g, and glycine 8g. The Chinese medicinal herb extract is prepared from epimeddium, morinda root, dodder seed and and licorice root.

Description

technical field [0001] The invention relates to a diluent of frozen semen in the improvement of livestock artificial insemination technology, in particular to a diluent of Chinese herbal medicine for bovine frozen semen. Background technique [0002] In 1780, the Italian zoologist Spallanznni put dog semen into snow for freezing, and observed the activity of sperm after thawing at room temperature, thus starting the research on the preservation of semen below 0°C. At the beginning of the 20th century, the research on semen freezing with refrigeration technology or artificial solid gas began to appear, and gradually attracted the attention of scientists from all over the world. In 1908, Maximov immersed plant tissues and cells in glycerol solution and cooled the temperature to -22°C. It was found that some cells survived. This was the first report of using a cryoprotectant. In 1937, Bernslain et al. used 9.2% (1M) glycerol solution to successfully pre...

AI Technical Summary

Problems solved by technology

However, there are very few reports on the effect of adding Chinese herbal medicine extracts directly to the semen dilution to study the effect on frozen semen of livestock, and there are almost no reports on the frozen semen of cattle.