Production of gene carrier and use thereof

A technology of gene carriers and complexes, applied in the field of preparation of gene carrier materials, can solve the problems of non-targeting and low efficiency, achieve good biocompatibility, satisfy effectiveness and safety, and have the effect of wide source of raw materials

Inactive Publication Date: 2005-12-28
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Non-viral vectors still face many challenges. For example, although liposomes have high gene transfection efficiency at some cell levels, even if they are injected into tumors in vivo, their efficiency is still low and they have no targeting.

Method used

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  • Production of gene carrier and use thereof
  • Production of gene carrier and use thereof
  • Production of gene carrier and use thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0036] ①Preparation of hydroxyapatite nanopowder

[0037] Analytical pure Ca(NO 3 ) 2 and (NH 4 )H 2 PO 4 respectively dissolved in deionized water to make a 1mol / L solution, Ca(NO 3 ) 2 and (NH 4 )H 2 PO 4 The solutions were mixed at a volume ratio of 5:3 at a rotational speed of 1500 rpm, and the pH value of the solution was adjusted to a range of 11-12 with NaOH, stirred for 10 hours, and then aged for 24 hours. The obtained precipitate was then put into an autoclave, treated at 200° C., 200 mv, and 200 rpm for 4 hours, and then washed, filtered, and dried to obtain hydroxyapatite nanoparticles.

[0038] ② Preparation of hydroxyapatite nanoparticle suspension

[0039] Take 0.5g of hydroxyapatite nanoparticles, put them into a centrifuge tube, add 20ml of deionized water, the concentration is 25mg / ml, disperse with ultrasonic wave (UltrasonicHemogenizer-4710, USA) for 60min, let stand for observation for 2h, the hydroxyapatite nanoparticles The suspension is milky...

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Abstract

A process for preparing the gene carrier includes such steps as proportionally mixing Ca(NO3)2 solution with (NH4)H2PO4 solution at 1500 rpm, and treating the obtained deposit at 200 deg.C and 200 rpm under 200 mv for 4 hr to obtain hydroxy apatite nanoparticles, which can be used as biologic ceramic material with high biocompatibility.

Description

Technical field: [0001] The invention relates to a material used in the medical field, in particular to a preparation method and application of a gene carrier material. Background technique: [0002] Gene therapy provides broad therapeutic prospects for many important human diseases such as genetic diseases, tumors, cardiovascular diseases and neurodegenerative diseases. Gene therapy is a means to treat diseases by introducing exogenous target genes into the body through vectors or other channels and displaying corresponding biological effects in target tissues and cells, and effective and safe therapeutic vectors are still The subject of hard work. [0003] Validity: [0004] (1) The mediated target gene can efficiently enter the desired target cells; [0005] (2) Not destroyed by various complements and various enzymes in plasma or tissue cells; [0006] (3) The transduced gene can pass through the nuclear membrane, enter the nucleus, and integrate into the chromosomal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00
Inventor 周科朝朱晒红黄苏萍李志友
Owner CENT SOUTH UNIV
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