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Use of 2,2'-dipyridine N-oxide disulfur for preparing anti-tumor drug

An anti-tumor drug, bipyridine technology, applied in 2 fields, can solve the problem that PTS2 anti-tumor application has not yet been discovered, and achieve the effect of small cytotoxicity, low onset dose, and obvious poisonous effect

Inactive Publication Date: 2005-12-21
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no discovery of the application of PTS2 in anti-tumor

Method used

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  • Use of 2,2'-dipyridine N-oxide disulfur for preparing anti-tumor drug
  • Use of 2,2'-dipyridine N-oxide disulfur for preparing anti-tumor drug
  • Use of 2,2'-dipyridine N-oxide disulfur for preparing anti-tumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: MTT method detects the killing effect of PTS2 on tumor cells

[0048] 1) Experimental principle:

[0049] Viable cells, especially proliferating cells, undergo mitochondrial hydrolysis to decompose MTT (a light yellow azole nitrogen salt) into blue-purple formazan (formaZan) crystals for color development, and use a microplate reader to measure the absorbance at specific wavelengths, and its optical density The value can reflect the proliferation of cells. MTT method is a fast, simple and semi-automatic method for quantitative analysis of cell proliferation and drug cytotoxicity.

[0050] 2) Experimental instruments and materials:

[0051] ELX-800 microplate reader was purchased from BIO-TEC company

[0052] PTS2 was prepared as a 5 mg / ml solution in DMSO before use

[0053] MTT (Sigma) is used to prepare a 5mg / ml solution with 1XPBS

[0054] DMSO (Amresco)

[0055] Culture medium: DMEM (GIBCO) RPMI1640 (GIBCO)

[0056] Serum: Four Seasons Green Fetal ...

Embodiment 2

[0069] Example 2: Hoechst staining method to detect the apoptosis of tumor cells after treatment with PTS2

[0070] 1) Experimental principle:

[0071] Hoechst33258 is a reactive dye that specifically binds to DNA. The binding of DNA is non-embedded and mainly binds to the A-T base region of DNA. Emits bright blue fluorescence when excited by UV light. It can enter the normal cell membrane without too much cytotoxic effect on the cells. It is a fast and simple method for the detection of apoptosis.

[0072] 2) Experimental instruments and materials

[0073] Nikon fluorescence microscope

[0074] Hoechst33258 (Sigma) prepared as a 1mg / ml solution when used

[0075] Fixative preparation: methanol: acetic acid = 3:1

[0076] Culture medium: DMEM (GIBCO) RPMI1640 (GIBCO)

[0077] Serum: Four Seasons Green Fetal Bovine Serum

[0078] Cell lines: HEK293, Hela, KB, 231, U937, K562 were purchased from Shanghai Cell Institute, Chinese Academy of Sciences

[0079] 3) Experiment...

Embodiment 3

[0082] Embodiment 3: MTT method compares the poisonous effect of PTS2 and PT, cisplatin and doxorubicin on tumor cells

[0083] 1) Experimental materials:

[0084] ELX-800 microplate reader was purchased from BIO-TEC company

[0085] PTS2 was prepared as a 5 mg / ml solution in DMSO before use

[0086] MTT (Sigma) is used to prepare a 5mg / ml solution with 1XPBS

[0087] DMSO (Amresco)

[0088] Cisplatin (Qilu Pharmaceutical Co., Ltd.) is prepared as a 5mg / ml solution with DMSO when used

[0089] Doxorubicin (Zhejiang Hisun Pharmaceutical Co., Ltd.) is used to prepare a 5mg / ml solution with DMSO

[0090] Culture medium: DMEM (GIBCO) RPMI1640 (GIBCO)

[0091] Serum: Four Seasons Green Fetal Bovine Serum

[0092] 96-well plate (Greiner)

[0093] The cell line Hela was purchased from Shanghai Cell Institute of Chinese Academy of Sciences

[0094] 2) Experimental steps:

[0095] Hela cells were seeded in 96-well plates at a density of 5000-10000 cells / well. Cells at 37°C, 5...

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Abstract

The present invention relates to a new application of 2.2'-dipyridine N-oxide disulfur in preparation of antitumor medicine. 2.2'-dipyridine N-oxide disulfur can effectively inhibit and kill tumor cell, and its cytotoxity for normal cell is low, relatively to currently frequently-used antitumor medicine cisplatin and doxorubicin its action for poisoning tumor cell is more obvious, it can be used for preparing medicine for clinically-curing tumor.

Description

technical field [0001] The invention relates to a new application of 2,2'-dipyridine N-oxide disulfide, in particular to the application of 2,2'-dipyridine N-oxide disulfide in the preparation of antitumor drugs. Background technique [0002] From the 1970s to the 1980s, the incidence of malignant tumors in my country increased significantly, and tumors became one of the major diseases that seriously endanger human life. According to the relevant statistics of the Ministry of Health, in my country, there are 2 million new cancer patients every year, and the number of deaths due to cancer is as high as 1.3 million per year. At present, the total number of cancer patients in the country has reached 4.5 million. As the population ages, smoking increases, physical activity decreases, and bad habits such as lack of vegetables and fruits in the diet may further lead to a surge in the number of cancer patients. A research report recently published by the International Cancer Resea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/444
Inventor 殷志敏牛德仲蒋继宏樊玉梅
Owner NANJING NORMAL UNIVERSITY
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