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Culture system, detection and analysis system and detection method for cancer cell colonies

A technology of culture system and analysis method, which is applied in the culture system of cancerous cell colonies and the system field of detection of cancer cell colonies, which can solve the problems of accuracy, inability to obtain the number of cell colonies, long culture time, etc.

Inactive Publication Date: 2005-10-05
侯德兴 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there are problems in the concentration and thickness of the agar medium in the prior art, and a stable number of cell colonies cannot be obtained
Moreover, the cultivation time is longer, requiring 20 to 30 days
Moreover, in the prior art, the detection of colonies can be observed with the naked eye under a microscope, although the number of colonies can be calculated, but the size and distribution of colonies cannot be analyzed
Moreover, counting the number of colonies requires a lot of time and physical strength. At the same time, the counting results will vary between individuals due to different experimenters, so there are problems in accuracy, which has become a problem to be solved by this test method

Method used

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  • Culture system, detection and analysis system and detection method for cancer cell colonies
  • Culture system, detection and analysis system and detection method for cancer cell colonies
  • Culture system, detection and analysis system and detection method for cancer cell colonies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] In Example 1, the culture system 1 was prepared by adding potato juice concentrate and TPA to the agar in the bottom layer 11 . Cultivate the culture system 1 under the specified conditions, in figure 2The middle represents the results of detection and analysis using the cancer cell colony detection and analysis system of the present invention. figure 2 In the graph shown in , the vertical axis represents the canceration inhibition rate, and the horizontal axis represents the concentration of potato juice, and the canceration inhibition rate of each concentration of potato juice is plotted.

[0074] according to figure 2 From the graph shown in , it can be known that if the concentration of potato juice increases, the rate of inhibition of carcinogenesis is improved.

Embodiment 2

[0076] In Example 2, the culture system 1 was prepared by adding blueberry anthocyanins contained in blueberries to the agar of the bottom layer 11 . Cultivate the culture system 1 under the specified conditions, in image 3 The middle represents the results of detection and analysis using the cancer cell colony detection and analysis system of the present invention. image 3 The graph shown in the figure 2 The curve shown in Figure 1 Like this, wherein the vertical axis represents the canceration inhibition rate, and the horizontal axis represents the blueberry anthocyanin concentration, and the canceration inhibition rate of each concentration of blueberry anthocyanin is plotted.

[0077] According to this graph, it can be known that if the concentration of blueberry anthocyanin increases, the rate of inhibition of carcinogenesis is improved.

Embodiment 3

[0079] In Example 3, the culture system 1 was prepared by adding rhein derived from rhubarb in traditional Chinese medicine to the agar in the bottom layer 11 . Cultivate the culture system 1 under the specified conditions, in Figure 4 The middle represents the results of detection and analysis using the cancer cell colony detection and analysis system of the present invention. Figure 4 The graph shown in the figure 2 The curve shown in Figure 1 Like, wherein the vertical axis represents the canceration inhibition rate, the horizontal axis represents the rhein concentration, and draws the canceration inhibition rate of each concentration of rhein.

[0080] From this graph, it can be seen that when the concentration of rhein derived from rhubarb increases, the carcinogenesis inhibition rate increases.

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Abstract

It is intended to provide a detection and analysis system for cancer cell colonies, whereby an environmental cell carcinogen causing cell carcinogenesis or a chemical or a food inhibiting cell carcinogenesis can be quickly and accurately detected, and a method therefor. Namely, a detection and analysis system for cancer cell colonies using a culture system (1) prepared by the agar overlaying method which comprises a bottom layer (1) being composed of a culture medium, soft agar and a carcinogenesis inducer and / or an anticancer agent and having a definite size and a top layer (12) being composed of a culture medium, soft agar and cells, and further provided with an optical microscope (21), an electronic data conversion unit (22) such as a digital camera and a computer system (23) for processing the data converted by the electronic data conversion unit (22). This computer system (23) has an image analysis software stored therein whereby the electronic data are grayed, calibrated and binarized by subtraction and single threshold and thus the presence or absence of colonies, the number of colonies, the size distribution of colonies, etc. are analyzed.

Description

technical field [0001] The invention relates to a detection and analysis system for cancerous cell colonies, a method for detecting cancerous cell colonies, and a culture system for cancerous cell colonies used in the detection method for cancerous cell colonies. More specifically, the present invention relates to a culture system comprising soft agar and implemented by the soft agar multilayer method, and a system for semi-automating cell culture using the culture system and the detection of cancer cell colonies by cultured cells, and its method. Background technique [0002] Although medical technology has made remarkable progress, cancer has remained the leading cause of death in Japan since 1981. Therefore, the whole society began to pay attention to the prevention of cancer. Therefore, we must first understand the process of cancer on the basis of better prevention of cancer. [0003] Usually, when carcinogenic substances remain in the body, most of the carcinogenic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5005C12M1/34C12Q1/04G01N33/15G01N33/50
Inventor 侯德兴藤井信
Owner 侯德兴
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