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Retrovirus carrier of bone morphogenetic protein 4 gene and its use

A retrovirus and vector technology, applied in the field of bone tissue genetic engineering, can solve the problems of low transduction efficiency and low cost, and achieve the effects of convenient collection, easy cultivation, and abundant sources

Inactive Publication Date: 2005-09-07
上海第二医科大学附属第九人民医院
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Problems solved by technology

The carriers of BMP gene transfer have their own characteristics. Retrovirus can integrate the target gene into the target cell genome and express it for a long time. Adenovirus can efficiently transfer regardless of the proliferation status of the target cell, and the mediated gene transfer is non-integrated. However, the currently constructed vectors contain many genes encoding proteins, and their products are highly immunogenic; the direct transduction method of naked DNA is simple, non-immunogenic, safe, and low in cost. The disadvantage is that the transduction efficiency is low

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  • Retrovirus carrier of bone morphogenetic protein 4 gene and its use
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  • Retrovirus carrier of bone morphogenetic protein 4 gene and its use

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Embodiment Construction

[0015] The retroviral vector of bone morphogenic protein 4 gene and its application are further described in detail in combination with specific experiments and analysis of their results and the accompanying drawings. The invention relates to the technical field of bone tissue genetic engineering, in particular to a recombinant vector containing bone morphogenic protein 4 gene, the vector transforms packaged virus particles, and utilizes the virus particles to prepare tissue-engineered bone and its application. Select a new type of retroviral expression vector pLEGFP, which is a green fluorescent expression system driven by the Moloney mouse leukemia virus promoter (MoMuLV). After transfecting packaging cells, it can transiently or stably express the packaging signal Ψ, and insert the target gene and marker Gene neo, the packaged progeny virus particles are secreted from the packaging cell membrane to the culture supernatant in the way of budding, which is infectious but has no...

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Abstract

The invention relates to the bone tissue gene engineering technical sphere, which in detail to recombinant vector of bone formation protein gene 4, the virus granule which converted and packed by said vector and preparing tissue engineering bone by applying the virus granule and its application. The invention is characterized in that: successfully constructing pLEGFP-hBMP-4 reverse transcript viral vectors by taking pLEGFP as the reverse transcript viral vectors; getting LEGFP-hBMP-4 and LEGP reverse transcript viral vectors by packing the cell PA317; constructing tissue engineering bone by using the marrow base cell modified by gene hBMP-4 as the seed cell.

Description

technical field [0001] The invention relates to the technical field of bone tissue genetic engineering, in particular to a recombinant vector containing bone morphogenic protein 4 gene, the vector transforms packaged virus particles, and utilizes the virus particles to prepare tissue-engineered bone and its application. Background technique [0002] Bone morphogenetic proteins (BMPs) belong to the superfamily of transforming growth factors, among which BMPs 2, 4, 5, 6, and 7 are closely related to the formation of bone, cartilage, tendon, periodontal tissue, and dentin. Osteogenic induction. However, the traditional BMP protein preparation, whether it is purified from bone matrix or expressed by genetic engineering, has complex procedures, low yield, and unstable activity; when repairing large-scale bone defects, the amount of BMP used is large, which may cause toxic reactions; BMP implanted in the body as an exogenous protein may also cause an immune response. In recent y...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/28C12N15/867
Inventor 蒋欣泉张志愿陈建国常庆王丽珍潘红芽袁荣涛张秀丽张濒
Owner 上海第二医科大学附属第九人民医院
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