Frozen semen of animal and its making method

A technology of freezing semen and its production method, which is applied in the fields of human or animal body preservation, animal delivery, medical science, etc., which can solve problems such as heavy workload and troublesome addition, and achieve the effect of increasing the conception rate

Inactive Publication Date: 2005-08-24
辽宁省辽宁绒山羊原种场有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of preservation of frozen semen diluent, it is generally prepared and used immediately, and cannot exceed 3 days at most. Therefore, frequent preparation of diluent becomes the main task of producing frozen semen, and it is troublesome to add some substances that are beneficial to the effect of conception during insemination. , heavy workload

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Diluent preparation:

[0028] Base solution: Base solution: Take 5.1g of lactose, 3.5g of glucose, 1.4g of sodium citrate dihydrate, 0.12g of tris, and 0.03g of EDTA, then add 100ml of double distilled water, boil the solution for disinfection, and cool down to 37°C;

[0029] Solution I: Take 80ml of base solution, add 20ml of fresh egg yolk, 100,000 iu of penicillin, and 0.1g of streptomycin;

[0030] Solution II: take 22ml of solution I, add 4.2ml of ethylene glycol, 5mg of testosterone, 130mg of glucose, and 12ml of VB12 (1ml:0.5mg).

[0031] Liquid I and liquid II were filtered with precision filter paper, and the filtrate was taken.

[0032] Storage of diluent: Divide prepared liquid I and liquid II into glass test tubes or other glass containers, and then freeze at -10°C. Each time the semen is processed, a certain amount of liquid I and liquid II is taken out and melted at a temperature below 37°C before use. But the diluent after thawing cannot be frozen agai...

Embodiment 2

[0040] Diluent preparation:

[0041] Base solution: Take 5.1g of lactose, 3.5g of glucose, 1.4g of sodium citrate dihydrate, 0.12g of tris, and 0.03g of EDTA, then add 100ml of double distilled water, boil and sterilize the solution, and cool down to 37°C.

[0042] Solution I: Take 80ml of base solution, add 20ml of fresh egg yolk, 100,000 iu of penicillin, and 0.1g of streptomycin.

[0043] Solution II: take 22ml of solution I, add 4.2ml of ethylene glycol, 10mg of testosterone, 130mg of glucose, and 24ml of VB12 (1ml:0.5mg).

[0044] Liquid I and liquid II were filtered with precision filter paper, and the filtrate was taken.

[0045] Storage of diluent: Divide prepared liquid I and liquid II into glass test tubes or other glass containers, and then freeze at -10°C. Each time the semen is processed, a certain amount of liquid I and liquid II is taken out and melted at a temperature below 37°C before use. But the diluent after thawing cannot be frozen again for the second ...

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PUM

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Abstract

A frozen semen of animal features that it contains animal semen, saccharide source, trace elements, antibiotic, ethanediol, testosterone, yolk and VB12. Its advantage is high semen activity. Its preparing process is also disclosed.

Description

Technical field: [0001] The invention relates to a seminal fluid freezing technology, which is widely applicable to animal seminal fluid freezing. Background technique: [0002] Today, with the rapid development of biotechnology, artificial insemination of the semen of excellent male animals can be vigorously promoted. However, due to geographical restrictions, under low temperature storage, the fresh semen survives and has the ability to fertilize for no more than 48 hours. Semen can be stored for decades after freezing, and it still has the ability to fertilize after thawing. For a long time, glycerin has been used as an antifreeze agent in the freezing of animal semen, but glycerol has a relatively large toxic and side effect on sperm, which affects the conception effect. The survival time of animal frozen semen after thawing is more than 10 hours shorter than that of fresh semen, which is also an important factor for the low conception rate of frozen semen. In terms of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00A61D19/00
Inventor 张世伟宋先忱朱延旭刘兴伟郭春润
Owner 辽宁省辽宁绒山羊原种场有限公司
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