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Molecule label linked with rice anti-rice blast gene

A rice blast resistance gene and molecular marker technology, applied in genetic engineering, plant genetic improvement, biochemical equipment and methods, etc., can solve problems such as inconvenient breeding procedures and complicated operation procedures, save time and cost, and achieve clear selection goals. Effect

Inactive Publication Date: 2005-08-03
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Since such markers require complicated procedures, expensive biochemical reagents, and a large amount of sample DNA, they are inconvenient for breeding programs

Method used

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Embodiment Construction

[0015] The implementation procedure of the present invention is: using the recombinant inbred line of Heijiazijaponica / Lijiang Xintuan Heigu constructed from 1996 to 1999 to inoculate three ZG1, one ZC5, one ZB15 race and the Japanese identification strain Bei 1 , Yan 54-04, P-2b and other different blast fungus (all known and public race strains), identified 8 different blast resistance genes, and we screened 2 of them with linked molecular markers. The disease resistance gene Pi-hk1(t) was identified in Bei 1 strain, and the disease resistance gene Pi-hk2(t) was identified in ZB15 race. (Wang Jianfei, He Xinjian, Zhang Hongsheng, etc. Genetic analysis of resistance to rice blast of japonica rice landrace Heikezijaponica in Taihu Lake Basin. Acta Genetica Sinica 2002, 29(9): 803-807)

[0016] In 2001, Heikuzi japonica was crossed with the susceptible variety Su Yunuo, and at the end of the year, F 1 The seeds were sent to Hainan Island for additional generations, and self-cr...

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Abstract

The present invention relates to molecular label linked with rice blast resisting gene and specially for the screening of rice blast resisting gene. Single plant DNA's of black hull japonica rice, Suyu glutinous rice and their F1 and F2 separated colony are extracted in SDS process; 350 pairs of SSR molecular labels are adopted in polymorphism screening of the amplified products of two parents and their F1; and the individuals of F2 anti-infective pond and F2 colony are screened for rice blast resisting gene linked label. It is found that the SSR labels RM144H240+260, RM7654H120, RM7212H120 and RM5923H150 in the long arm end of 11-th chromosome are linked with rice blast resisting gene Pi-hkl(t); and the SSR labels RM277H70, RM511H80+90, and RM2972H90 in the 12-th chromosome are linked with rice blast resisting gene Pi-hk2(t). The present invention can target the rice blast resisting gene accurately and raise the rice blast resisting selectively breeding efficiency greatly.

Description

1. Technical field [0001] The invention discloses molecular markers linked with rice blast resistance genes Pi-hk1(t) and Pi-hk2(t), which are used for molecular breeding of improving rice blast resistance and are specially used for screening rice blast resistance genes . 2. Technical background [0002] Rice is a vital food crop for global human growth in the 21st century. Rice diseases have robbed a considerable part of rice production, among which rice blast damage accounts for more than 70% of losses, and this damage is not only in developing countries, but also occurs in rice-producing countries or regions in the world. In the years when rice blast is prevalent, the yield loss is generally 10-20%, and it can reach more than 50% in serious cases. Some fields even fail to harvest, and lead to a decline in rice quality [Ling Zhong Zhuan. Rice Blast Resistance Breeding. Fujian Science and Technology Press, 1990]. The average annual occurrence area of ​​rice blast in my c...

Claims

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Application Information

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IPC IPC(8): A01H1/02C12N15/29C12Q1/68
Inventor 张红生王建飞黄骥王州飞李培富
Owner NANJING AGRICULTURAL UNIVERSITY
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