Method for obtaining high quality fertilized egg of scophthatmus maximus
A technology of turbot and fertilized eggs, applied in fish farming, application, climate change adaptation, etc., can solve the problems that have not been raised, obtain high-quality fertilized eggs comprehensive technology, and achieve the effect of improving quality
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[0018] Turbot is a newly introduced high-quality mariculture species, which has developed rapidly, but the technology is not yet perfect. During the seed production of turbot, the quality of eggs and the fertilization rate are extremely unstable, and the fertilization rate is less than 10% when it is low, which seriously affects the production of turbot seed. Through the adoption of comprehensive measures, the quality of turbot eggs and fertilized eggs was improved, and the fertilization success rate was 89.6%.
[0019] Describe technical content of the present invention in detail below by embodiment:
[0020] This study was carried out at the seawater fish breeding test site in Penglai City, Shandong Province, and comprehensive technical measures were implemented. The specific process is as follows:
[0021] Firstly, the cultivation of turbot broodstock and the method of artificial egg insemination were determined, and then the large-scale breeding experiment of broodstock c...
Embodiment 1
[0022] Embodiment 1, the cultivation of turbot broodstock and the determination of ovum collection and insemination method:
[0023] 1. Determination of broodstock breeding methods:
[0024] A total of 40 broodstock of turbot (Scophthalmus maximus L.) were selected in the experiment, and they were divided into 4 different experimental groups, with 4 females and 6 males in each group. There are 3 parallels in each group. The broodstock is the preferred healthy turbot with normal body color. The back of the normal body color turbot is blue-brown with bony protrusions, and the ventral surface is smooth and white. Age of broodstock: Both female and male are 3 years old. The average size is 2650±12.0g in weight and 45±4cm in total length. Placed in 12 20m3 cement ponds for flowing water aerated culture, the water for cultivation is ground seawater and natural seawater, mixed with sand after filtration, the annual water quality changes are: DO>6.0mg / L, pH=7.8-8.2, S=30-32, T=8-1...
Embodiment 2
[0095] Embodiment two, carry out the large-scale breeding test of broodstock cultivation and artificial insemination.
[0096] 240 broodstock of turbot (Scophthalmus maximus L.), which are preferably healthy turbot with normal body color. Age of broodstock: Both female and male are 3 years old. The average size is 2700±15.0g in weight and 46±4cm in total length. Placed in 8 20m3 cement ponds for flowing water aerated culture. The water used for culture is underground seawater and natural seawater, which are mixed with sand after filtration. The changes in water quality throughout the year are: DO>6.0mg / L, pH=7.8-8.2, S=30-32, T=8-18°C.
[0097]Feed and feeding of broodstock: The feed of broodstock is a prepared artificial compound feed, which is a semi-moist pellet feed prepared from the following feed materials and dosage (in 100 kg of ingredients): 60 kg of fresh defatted fish meal, 10 kg of soybean meal , 10 kg of peanut meal, 4 kg of feed yeast, 2 kg of compound multidi...
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