Lichen bacillus strain (B-0A12) and preparation thereof

Abstract

One strain for preventing and controlling root rot caused by soybean cytocyst nematosis and fusarium is named as Bucillus licheniformis CGMCC No. 1035. The liquid bacteria preparation for preventing and controlling root rot caused by soybean cytocyst nematosis and fusarium is prepared through inoculation with the said strain, fermentation and addition of additive. The fermentation process has temperature of 27-35 deg.c and pH 6.8-8.0; and the bacteria content in finishing fermentation is 700 million / ml. Corresponding solid preparation and seed coating preparation may be also prepared. The preparation for preventing and controlling soybean cytocyst nematosis and fusarium can also regulate microbial ecologic system around root system, promote plant growth and increase yield.

Description

technical field [0001] The invention relates to the field of microorganisms, more specifically, relates to a bacillus licheniformis bacterial strain and a preparation thereof for preventing and treating plant root diseases, in particular for preventing and treating soybean cyst nematode and root rot. technical background [0002] Soybean originated in my country. With the expansion of planting area, the problem of repeated cropping of soybeans has become more and more serious. Among them, the incidence area of ​​soybean cyst nematode and root rot accounted for more than 70% of the soybean planting area, and the loss reached 10-40%. The emergence of serious plots was irregular, the plants were short and yellow, commonly known as "fire dragon seedlings", and even Lead to extinct production, and the incidence area has an increasing trend in recent years. [0003] At present, the effective chemical drugs against soybean cyst nematodes and root rot are still using carbofuran and...

AI Technical Summary

Problems solved by technology

[0004] There are many domestic and foreign research reports on the application of nematode fungi and parasitic bacteria to control plant parasitic nematodes (Lin Maosong, 1990; Liu Xingzhong et al., 1991, 1992), and the fungi Pochonia chlamydosporum and Paecilomyceslilacinus for preventing and controlling soybean cyst nematodes have entered field experiments (Liu Xingzhong, Patent No.: ZL 951 21114.5; Pan Cangsang, Patent No.: CN1149393, CN1149394; Liu Weizhi, Patent No.: CN1225236), but due to the characteristics of the fungus itself, its application is limited. The fermentation time usually takes 5-7 days. If liquid-solid two-phase fermentation is adopted, the time will be increased to 10-20 days (Pan Cangsang), which will increase the cost

2) The storage period is short. Although the liquid bacterial agent is beneficial for seed treatment during large-scale planting in the Northeast, the storage time is short, only 3-6 months, which is not conducive to commercialization

3) Liquid-solid two-phase fermentation, although the storage period of the preparation can be extended to 18 months, but the liquid-solid two-phase fermentation time is greatly extended

There is no research report on the application of Bacillus licheniformis to control soybean cyst nematode and soybean root rot caused by Fusarium