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Use for combined detecting multiple virus microbe label by biological chip and mass analyzer

A biochip and biomarker technology, applied in biological testing, material analysis by measuring secondary emissions, instruments, etc., can solve the problem that disease markers cannot be detected at the same time

Inactive Publication Date: 2005-05-11
许洋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using antibodies and three-color fluorescence, up to three disease markers can be detected, but more than three disease markers cannot be detected at the same time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Normal people, HBV, HCV, HIV, the distinction of syphilis (TP) microorganisms in blood biomarkers

[0018] (1) Experimental method

[0019] 1. Materials

[0020]1. Specimen source: collect serum / plasma samples from clinically diagnosed patients. 70 cases of control group (normal people), 71 cases of HBV patients, 45 cases of HCV patients, 30 cases of HIV patients, 10 cases of syphilis patients.

[0021] 2. Reagents: Acetonitrile, trifluoroacetic acid, and SINAPINIC acid (Sinapinic acid) were all purchased from Sigma.

[0022] 2. Method

[0023] 1. Collection of samples: absorb serum after whole blood collection and store at -80°C;

[0024] 2. Sample preparation: HBV positive serum, HCV positive serum, HIV positive serum, syphilis positive serum.

[0025] 3. Pretreatment of the chip: 100 μl of the diluted sample was loaded into a groove covering one chip site and shaken slightly for 0.5 hour at room temperature. Remove buffer from tank. 200 μl of the sam...

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Abstract

A method of capturing multiple virus germ biological label at biological chip absorption surface and carrying out analysis to captured biological label can be used to detect multiple biological label group such as hepatitis B, hepatitis C, Aide and syphilis in blood in order to identify normal person or person with abovesaid disease.

Description

technical field [0001] The invention relates to a new method for analyzing proteins in biological samples, a biomarker captured by Protein A and Protein G adsorption surface chips, and using laser analysis ionization mass spectrometry to detect the biomarker. The invention mentioned here is generally related to hepatitis B (hepatitis B virus, HBV), hepatitis C (hepatitis C virus, HCV), AIDS (human immunodeficiency virus, HIV), syphilis (treponema pallidum, TP) microorganisms related to the field of research. More specifically, this invention relates to biomarkers, and these antigens or biomarkers can be used to distinguish hepatitis B, hepatitis C, AIDS, and syphilis at one time with higher specificity and sensitivity. The present invention can be applied to screening in blood banks or hospitals. Background technique [0002] Both normal function and pathological characteristics of cells depend to some extent on the function of proteins expressed by cells. Therefore, iden...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/22G01N33/532G01N33/569G01N33/576G01N33/58G01N33/68
Inventor 许洋
Owner 许洋
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