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Culturing method of microbe for high temperature waste water biochemical treatment

A cultivation method and high-temperature wastewater technology, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve problems such as cumbersome methods and difficult mastery

Inactive Publication Date: 2005-03-23
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN1144535A discloses the method for cultivating microorganisms, by cultivating, mutating and stabilizing microorganisms in a mutation fermentation container containing a substrate rich in nutrients at the same time, microorganisms for decomposing harmful substances can be quickly cultivated, but the method cumbersome, difficult to master

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A kind of terrestrial oil production wastewater, COD is 656mg / L, temperature is 55°C.

[0024] For the collection of strains, the activated sludge is collected from the ordinary biochemical aeration tank, and the pure activated sludge is separated after filtering, settling, and discarding the supernatant. The activated sludge is fresh and the color is yellowish brown.

[0025] Add oil extraction wastewater with a temperature of 25°C and a COD content of 656mg / L to the active sewage, and add urea and disodium hydrogen phosphate at a ratio of COD:N:P=200:5:1 (weight ratio) as microbial growth and reproduction Nitrogen source and phosphorus source; add glucose solution according to the ratio of 1:1 of glucose performance COD and wastewater COD; add microbial growth promoter at a concentration of 4mg / L. Control the dissolved oxygen to 0.6 mg / L for aeration for 2 days, and discharge the supernatant after settling. After that, one cycle per day is used. The aeration process ...

Embodiment 2

[0027] A kind of waste water from oil production in offshore oilfields, with a COD of 493mg / L and a temperature of 58°C.

[0028] For the collection of strains, the activated sludge is collected from the ordinary biochemical aeration tank, and the pure activated sludge is separated after filtering, settling, and discarding the supernatant. The activated sludge is fresh and the color is yellowish brown.

[0029] Add oil extraction wastewater with a temperature of 23°C and a COD content of 493mg / L to the active sewage, and add urea and disodium hydrogen phosphate at a ratio of COD:N:P=200:10:1 (weight ratio) as microbial growth and reproduction Nitrogen source and phosphorus source; add glucose solution according to the ratio of 2:1 between glucose COD and wastewater COD; add microbial growth promoter at a concentration of 8mg / L. Control the dissolved oxygen to 0.6 mg / L for aeration for 3 days, and discharge the supernatant after settling. After that, one cycle per day is used....

Embodiment 3

[0031] A kind of wastewater discharged from a refinery, the temperature of the wastewater is 45°C, and the COD concentration of the wastewater is about 1000mg / L.

[0032] For the collection of strains, the activated sludge is collected from the ordinary biochemical aeration tank, and the pure activated sludge is separated after filtering, settling, and discarding the supernatant. The activated sludge is fresh and the color is yellowish brown.

[0033] Add oil refinery wastewater with a temperature of 22°C and a COD content of 1000 mg / L to the active sewage, and add urea and disodium hydrogen phosphate at a ratio of COD:N:P=200:6:1 (weight ratio) as microbial growth and reproduction Nitrogen source and phosphorus source; add glucose solution according to the ratio of 4:1 between the COD of glucose and the COD of wastewater; add microbial growth promoter at a concentration of 8mg / L. Aeration is carried out for 18 hours while the dissolved oxygen is controlled to be above 0.6 mg / ...

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Abstract

A process for culturing the microbes used to biochemically treat high-temp sewage includes such steps as choosing the active sludge in ordinary biochemical aeratnig pool as bacterial seeds, depositing, aerating, adding the sewage of oil field, removing supernatant, adding stabilizer of protein and growth promoter of microbes, and naturolizing.

Description

technical field [0001] The invention relates to a method for cultivating microorganisms, in particular to a method for cultivating microorganisms used to decompose organic pollutants in high-temperature wastewater, especially a method for cultivating microorganisms for treating high-temperature oil recovery wastewater. Background technique [0002] Microbiological treatment of sewage has the advantages of large treatment capacity, high purification rate, low energy consumption, simple operation, and no secondary pollution, and has been widely used in the field of sewage treatment. However, ordinary microorganisms can only be applied to the treatment of wastewater at normal temperature or medium temperature (generally below 35°C). Ordinary microorganisms cannot survive under high temperature conditions, and of course the purpose of purifying sewage cannot be achieved. [0003] In some industrial production, waste water with higher temperature will be discharged, but due to re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
Inventor 许谦林大泉王俊英
Owner CHINA PETROLEUM & CHEM CORP
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