Food allergens, method of detecting food allergens and method of detecting food allergy-inducing foods

Abstract

The present invention provides a method for detecting food allergens, antibodies and antigens to prepare the antibodies. The antigens of this invention are a mixture comprising multiple native and / or heated food allergens that IgE antibodies of food-allergy patients recognize. The antibodies of this invention are prepared by immunizing animals with the above-mentioned antigens. The food allergen-detecting method of this invention relates to the above-mentioned antibodies. As the method can detect food allergens and food allergy-inducing foods, it can provide safety to food allergy patients.

Description

Background of the invention [0001] The present invention relates to a mixture containing various natural and / or denatured allergens that can be recognized by IgE antibodies in food allergic patients, antibodies prepared by immunizing animals with the mixture, and using the antibodies to detect food allergens and induce allergic reactions way of food. The present invention provides safety to patients with food allergies, because the present invention helps to understand the mechanism of food allergic reactions, develop and test hypoallergenic foods, test food, food raw materials and products such as food preparation machines and Allergens in the environment such as food preparation processes. [0002] Food allergy is an unwanted immune response to an allergic reaction-inducing substance (hereinafter referred to as a food allergen) in food ingested by a susceptible individual. Food allergies can lead to dermatitis, asthma, digestive tract disorders, anaphylactic shock, etc. A...

AI Technical Summary

Problems solved by technology

However, all or some of these methods have one or more of the following deficiencies: (1) These methods cannot always detect antigens to which food allergic patients are sensitive

That is, they cannot always detect substances recognized by the patient's IgE antibodies; (2) these methods can detect known antigens, and each method can only detect one allergen (single antigen); ( 3) These methods using antibodies to detect a single antigen are not suitable for foods containing inhibitory substances; (4) As shown in the examples below, these methods using antibodies to detect a single antigen cannot be tested on foods that induce allergic reactions Accurate quantification; (5) These methods using antibodies to detect a single antigen are not suitable for the detection of foods that do not contain antigens that induce allergic reactions (such as methods using a single antibody prepared by contacting ovalbumin in egg whites are not applicable For checking egg yolk, mayonnaise, etc.

food anaphylaxis is known to be caused by egg yolk); (6) these methods using antibodies that detect a single antigen are not suitable for examining processed foods because they cannot detect denatured or molecularly modified allergens; and ( 7) Monoclonal antibodies against natural and denatured β-lactoglobulin, ovalbumin and α-casein have been reported (Allergy, Vol.50, p309)

However, these methods are less useful if epitopes are removed or altered by food processing because monoclonal antibodies can only detect a single epitope in a food allergen molecule

[0009] Although a method for screening food allergens using serum obtained from patients allergic to rice (Japanese Patent Publication 2000-65820), eggs and milk (Japanese Meat Science, vol.39, p166-169) has been reported, and in Can be used in hospitals but not in inspection groups or in food processing plants because these methods require large volumes of serum from patients

Although food antigen detection methods relying on allergic reactions have been reported (FFI J., No. 180, p77-82), complex methodology and laboratory animal husbandry make this method unsuitable for most food processing plants

Although methods using flow cytometry and enzyme-labeled antibodies and allergen sensors using microelectrodes have been established (Japan Food Industry, Vol.42, p53-56), many problems remain to be resolved before these methods can be put into practical use

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