Extracting method of propolis effective component and its use
An extraction method and technology of active ingredients, applied in the field of extraction of active ingredients in propolis, can solve problems such as no reports on extraction, and achieve the effects of simple extraction method, low extraction cost and simple composition
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[0015] The method of the present invention and the test results of related applications are explained in detail below.
[0016] 1. Extraction method of propolis
[0017] Take fresh propolis, put it in distilled water (W / W=1:20) and stir at room temperature for 48 hours after crushing; filter; add water to the filter residue and repeatedly extract and filter; combine the filtrates and centrifuge at 15,000rpm for 60 minutes;
[0018] The supernatants were combined; the water-soluble propolis fraction was obtained after freeze-drying.
[0019] It has been determined that the extracted water-soluble propolis contains three flavonoid components, see attached figure 1 . figure 1 It is the result of thin-layer scanning of flavonoids in propolis water extract under visible light wavelength, and the color developer used is 5% ferric chloride-ethanol solution.
[0020] Two, the use research of propolis extract of the present invention
[0021] Experimental methods and conditions:
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Abstract
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