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Agglutination reaction and separation vessel

A container and agglutinate technology, applied in the field of agglutination assay, can solve problems such as wrong results of agglutination assay

Inactive Publication Date: 2004-11-10
ORTHO-CLINICAL DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This can lead to erroneous results in agglutination assays

Method used

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  • Agglutination reaction and separation vessel
  • Agglutination reaction and separation vessel
  • Agglutination reaction and separation vessel

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0072] Will BIOVUE with plugin TM Columns were compared to columns without inserts in order to determine the effectiveness of each configuration for maintaining the spatial air barrier separating the reactants from the separation matrix during incubation. Utilize an insert with a 0.040 inch hole. 40 microliters of buffer was added to each of the 840 columns used in the experiment. The pipette was artificially used at an angle of approximately 45° to the vertical axis of the column in order to deliver the 40 microliters of solution. The column was then observed to determine if an air gap was maintained beneath the reaction chamber. Table 1 gives the number of "leaks out".

[0073] Number of experiments

example 2

[0075] Reagents were also added to the columns (with and without inserts) and incubated at 37°C for 10 minutes. To each of the 480 columns used in the test, 40 microliters of buffer, 40 microliters of serum and 10 microliters of erythrocyte suspension were added. Use the pipette at an angle of approximately 45° to pipette the reagents. After a period of incubation, the column is checked to determine whether an air gap remains under the reaction chamber. Table 2 gives the "leakage" frequencies.

[0076] Number of experiments

example 3

[0078] Fill the column with 40 microliters of buffer with an automatic pipette at an angle of approximately 45°. Automatic pipettes typically use higher transfer forces than manual methods. Observations were made after filling to determine whether an air gap remained under the reaction chamber. Table 3 presents the results for columns with and without inserts.

[0079] Number of experiments

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Abstract

A vessel for conducting blood cell agglutination assays is disclosed. A barrier retains reactants in an upper chamber during incubation, then, in response to a force, permits reagents to enter a lower chamber containing a matrix for separating agglutination.

Description

[0001] This application is a divisional application of a Chinese patent application with application number 97110916.8 and a filing date of February 2, 1997. [0002] Application 97110916.8 is a continuation-in-part of U.S. Patent Application No. 08 / 093,106, filed July 16, 1993, which is U.S. Patent Application No. 08 / 092,157, filed July 15, 1993 A follow-up application to a now-abandoned U.S. patent application. technical field [0003] The present invention relates to the field of agglutination assays, in particular to containers for performing agglutination assays and isolating agglutinates. [0004] Blood group serology requires the determination of hemocompatibility between blood donor and recipient prior to transfusion to a patient or organ transplantation. Cell compatibility is determined by demonstrating the absence of an immunological reaction between antibodies contained in the patient's serum and antigens in blood cells from the donor. [0005] Many different bloo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61JG01N33/48A61J1/05B01L3/00B01L3/14B65DG01NG01N33/00G01N33/50G01N33/53G01N33/536G01N33/537G01N33/538G01N33/558G01N33/80G01N33/86
CPCG01N33/5304G01N35/026
Inventor W·米尔彻尼奥斯基M·祖列克K·J·雷斯D·E·比尔托尔德L·戴维斯T·M·塞卡维杰D·M·戴维斯
Owner ORTHO-CLINICAL DIAGNOSTICS
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